Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86058
Title: WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels
Authors: Salvi, Amrita
Thanabalu, Thirumaran
Keywords: WIP
Actin Cytoskeleton
Issue Date: 2016
Source: Salvi, A., & Thanabalu, T. (2017). WIP promotes in-vitro invasion ability, anchorage independent growth and EMT progression of A549 lung adenocarcinoma cells by regulating RhoA levels. Biochemical and Biophysical Research Communications, 482(4), 1353-1359.
Series/Report no.: Biochemical and Biophysical Research Communications
Abstract: Cancer cell migration and invasion involves actin cytoskeleton reorganization, which is regulated by the WASP (Wiskott Aldrich Syndrome Protein) family of proteins such as WASP, N-WASP (Neural-WASP) and WASP interacting protein (WIP). In this study, we found that the expression of WIP was significantly upregulated in metastatic A5-RT3 cells compared to its parental non-tumorigenic HaCaT cells. Using A549 human lung adenocarcinoma cell line as the model system, we found that WIP regulates cell invasion, proliferation and anchorage-independent growth. Expression of WIP was enhanced during TGF-β1 induced epithelial-mesenchymal transition (EMT) and overexpression of WIP accelerated EMT while knocking down WIP attenuated EMT associated morphological changes. Knocking down WIP expression in A549 cells significantly reduced RhoA levels and WIP was found to interact with RhoA suggesting that WIP might be executing its function by regulating RhoA. Acquisition of invasive, proliferative properties and anoikis resistance is the central step in metastasis indicating a novel function of WIP in cancer progression.
URI: https://hdl.handle.net/10356/86058
http://hdl.handle.net/10220/43930
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2016.12.040
Rights: © 2016 Elsevier Inc.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SBS Journal Articles

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