Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86059
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMitra, Payalen
dc.contributor.authorThanabalu, Thirumaranen
dc.date.accessioned2017-10-19T03:24:59Zen
dc.date.accessioned2019-12-06T16:15:13Z-
dc.date.available2017-10-19T03:24:59Zen
dc.date.available2019-12-06T16:15:13Z-
dc.date.issued2016en
dc.identifier.citationMitra, P., & Thanabalu, T. (2016). Myogenic differentiation depends on the interplay of Grb2 and N-WASP. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1864(3), 487-497.en
dc.identifier.issn0167-4889en
dc.identifier.urihttps://hdl.handle.net/10356/86059-
dc.description.abstractMyogenesis requires a well-coordinated withdrawal from cell cycle, morphological changes and cell fusion mediated by actin cytoskeleton. Grb2 is an adaptor protein whose central SH2 domain binds to phosphorylated tyrosine residues of activated receptors and activates intracellular signaling pathway, while its N-terminal and C-terminal SH3 domains bind to proline rich proteins such as N-WASP (Neural-Wiskott Aldrich Syndrome Protein). We found that the expression of Grb2 was increased at the beginning of differentiation and remained constant during differentiation in C2C12 myoblasts. Knocking down endogenous Grb2 expression caused a significant increase in the fusion index and expression of MyHC, a terminal differentiation marker when compared with the control. Over expression of Grb2 in C2C12 (C2C12Grb2-Myc) reduced myotube formation and expression of MyHC. Similarly over expression of Grb2P49L-Myc (N-terminal SH3 domain mutant) or Grb2R86K-Myc (SH2 domain mutant) inhibited myogenic differentiation of C2C12 cells. However, the expression of Grb2P206L-Myc (C-terminal SH3 domain mutant) did not inhibit myotube formation and expression of MyHC. This suggests that the C-terminal SH3 domain of Grb2 is critical for the inhibition of myogenic differentiation. The C2C12Grb2-Myc cells have reduced phalloidin staining at late stages of differentiation. Expression of N-WASP in C2C12Grb2-Myc cells rescued the myogenic defect and increased phalloidin staining (increased F-actin) in these cells. Thus our results suggest that Grb2 is a negative regulator of myogenesis and reduces myogenic differentiation by inhibiting actin polymerization/remodeling through its C-terminal SH3 domain.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.language.isoenen
dc.relation.ispartofseriesBiochimica et Biophysica Acta (BBA) - Molecular Cell Researchen
dc.rights© 2016 Elsevier B.V.en
dc.subjectMyogenesisen
dc.subjectN-WASPen
dc.titleMyogenic differentiation depends on the interplay of Grb2 and N-WASPen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1016/j.bbamcr.2016.12.011en
item.fulltextNo Fulltext-
item.grantfulltextnone-
Appears in Collections:SBS Journal Articles

SCOPUSTM   
Citations 50

4
Updated on Nov 27, 2022

Web of ScienceTM
Citations 50

4
Updated on Dec 3, 2022

Page view(s) 50

415
Updated on Dec 8, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.