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Title: Reduced intracellular c-di-GMP content increases expression of quorum sensing-regulated genes in pseudomonas aeruginosa
Authors: Chua, Song Lin
Liu, Yang
Li, Yingying
Ting, Hui Jun
Kohli, Gurjeet S.
Cai, Zhao
Suwanchaikasem, Pipob
Goh, Kelvin Kau Kit
Ng, Sean Pin
Tolker-Nielsen, Tim
Yang, Liang
Givskov, Michael
Keywords: Pseudomonas Aeruginosa
Issue Date: 2017
Source: Chua, S. L., Liu, Y., Li, Y., Ting, H. J., Kohli, G. S., Cai, Z., et al. (2017). Reduced Intracellular c-di-GMP Content Increases Expression of Quorum Sensing-Regulated Genes in Pseudomonas aeruginosa. Frontiers in Cellular and Infection Microbiology, 7, 451-.
Series/Report no.: Frontiers in Cellular and Infection Microbiology
Abstract: Cyclic-di-GMP (c-di-GMP) is an intracellular secondary messenger which controls the biofilm life cycle in many bacterial species. High intracellular c-di-GMP content enhances biofilm formation via the reduction of motility and production of biofilm matrix, while low c-di-GMP content in biofilm cells leads to increased motility and biofilm dispersal. While the effect of high c-di-GMP levels on bacterial lifestyles is well studied, the physiology of cells at low c-di-GMP levels remains unclear. Here, we showed that Pseudomonas aeruginosa cells with high and low intracellular c-di-GMP contents possessed distinct transcriptome profiles. There were 535 genes being upregulated and 432 genes downregulated in cells with low c-di-GMP, as compared to cells with high c-di-GMP. Interestingly, both rhl and pqs quorum-sensing (QS) operons were expressed at higher levels in cells with low intracellular c-di-GMP content compared with cells with higher c-di-GMP content. The induced expression of pqs and rhl QS required a functional PqsR, the transcriptional regulator of pqs QS. Next, we observed increased production of pqs and rhl-regulated virulence factors, such as pyocyanin and rhamnolipids, in P. aeruginosa cells with low c-di-GMP levels, conferring them with increased intracellular survival rates and cytotoxicity against murine macrophages. Hence, our data suggested that low intracellular c-di-GMP levels in bacteria could induce QS-regulated virulence, in particular rhamnolipids that cripple the cellular components of the innate immune system.
DOI: 10.3389/fcimb.2017.00451
Rights: © 2017 Lin Chua, Liu, Li, Jun Ting, Kohli, Cai, Suwanchaikasem, Kau Kit Goh, Pin Ng, Tolker-Nielsen, Yang and Givskov. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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