Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86518
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dc.contributor.authorPiazza, Aurèleen
dc.contributor.authorCui, Xiaojieen
dc.contributor.authorAdrian, Michaelen
dc.contributor.authorSamazan, Frédéricen
dc.contributor.authorHeddi, Brahimen
dc.contributor.authorPhan, Anh-Tuanen
dc.contributor.authorNicolas, Alain Gen
dc.date.accessioned2017-11-15T07:39:20Zen
dc.date.accessioned2019-12-06T16:23:50Z-
dc.date.available2017-11-15T07:39:20Zen
dc.date.available2019-12-06T16:23:50Z-
dc.date.issued2017en
dc.identifier.citationPiazza, A., Cui, X., Adrian, M., Samazan, F., Heddi, B., Phan, A.-T., et al. (2017). Non-Canonical G-quadruplexes cause the hCEB1 minisatellite instability in Saccharomyces cerevisiae. eLife, 6, e26884-.en
dc.identifier.issn2050-084Xen
dc.identifier.urihttps://hdl.handle.net/10356/86518-
dc.description.abstractG-quadruplexes (G4) are polymorphic four-stranded structures formed by certain G-rich nucleic acids in vitro, but the sequence and structural features dictating their formation and function in vivo remains uncertain. Here we report a structure-function analysis of the complex hCEB1 G4-forming sequence. We isolated four G4 conformations in vitro, all of which bear unusual structural features: Form 1 bears a V-shaped loop and a snapback guanine; Form 2 contains a terminal G-triad; Form 3 bears a zero-nucleotide loop; and Form 4 is a zero-nucleotide loop monomer or an interlocked dimer. In vivo, Form 1 and Form 2 differently account for 2/3rd of the genomic instability of hCEB1 in two G4-stabilizing conditions. Form 3 and an unidentified form contribute to the remaining instability, while Form 4 has no detectable effect. This work underscores the structural polymorphisms originated from a single highly G-rich sequence and demonstrates the existence of non-canonical G4s in cells, thus broadening the definition of G4-forming sequences.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent38 p.en
dc.language.isoenen
dc.relation.ispartofserieseLifeen
dc.rights© 2017 Piazza et al (Published by eLife Sciences Publications). This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.en
dc.subjectSaccharomyces cerevisiaeen
dc.subjectG-quadruplexes (G4)en
dc.titleNon-Canonical G-quadruplexes cause the hCEB1 minisatellite instability in Saccharomyces cerevisiaeen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen
dc.identifier.doi10.7554/eLife.26884en
dc.description.versionPublished versionen
item.grantfulltextopen-
item.fulltextWith Fulltext-
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