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Title: Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation
Authors: Edupuganti, Raghu Ram
Harikumar, Arigela
Aaronson, Yair
Biran, Alva
Sailaja, Badi Sri
Nissim-Rafinia, Malka
Azad, Gajendra Kumar
Cohen, Malkiel A.
Park, Jung Eun
Shivalila, Chikdu S.
Markoulaki, Styliani
Sze, Siu Kwan
Jaenisch, Rudolf
Meshorer, Eran
Keywords: SET
Embryonic Stem Cells
Issue Date: 2017
Source: Edupuganti, R. R., Harikumar, A., Aaronson, Y., Biran, A., Sailaja, B. S., Nissim-Rafinia, M., et al. (2017). Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. Stem Cell Reports, 9(4), 1291-1303.
Series/Report no.: Stem Cell Reports
Abstract: Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage.
ISSN: 2213-6711
DOI: 10.1016/j.stemcr.2017.08.021
Rights: © 2017 The Authors. This is an open access article under the CC BY-NC-ND license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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