Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86614
Title: A broad range quorum sensing inhibitor working through sRNA inhibition
Authors: Stegger, Marc
Lorenzen, Frederik
Rybtke, Morten
Moscoso, Joana A.
Jakobsen, Tim H.
Warming, Anders N.
Vejborg, Rebecca M.
Andersen, Jens B.
Petersen, Rico
Andersen, Paal Skytt
Nielsen, Thomas Eiland
Tolker-Nielsen, Tim
Filloux, Alain
Ingmer, Hanne
Givskov, Michael
Keywords: Microbiology
Pathogenesis
Issue Date: 2017
Source: Jakobsen, T. H., Warming, A. N., Vejborg, R. M., Moscoso, J. A., Stegger, M., Lorenzen, F., Rybtke, M., et al. (2017). A broad range quorum sensing inhibitor working through sRNA inhibition. Scientific Reports, 7, 9857-.
Series/Report no.: Scientific Reports
Abstract: For the last decade, chemical control of bacterial virulence has received considerable attention. Ajoene, a sulfur-rich molecule from garlic has been shown to reduce expression of key quorum sensing regulated virulence factors in the opportunistic pathogen Pseudomonas aeruginosa. Here we show that the repressing effect of ajoene on quorum sensing occurs by inhibition of small regulatory RNAs (sRNA) in P. aeruginosa as well as in Staphylococcus aureus, another important human pathogen that employs quorum sensing to control virulence gene expression. Using various reporter constructs, we found that ajoene lowered expression of the sRNAs RsmY and RsmZ in P. aeruginosa and the small dual-function regulatory RNA, RNAIII in S. aureus, that controls expression of key virulence factors. We confirmed the modulation of RNAIII by RNA sequencing and found that the expression of many QS regulated genes encoding virulence factors such as hemolysins and proteases were lowered in the presence of ajoene in S. aureus. Importantly, our findings show that sRNAs across bacterial species potentially may qualify as targets of anti-virulence therapy and that ajoene could be a lead structure in search of broad-spectrum compounds transcending the Gram negative-positive borderline.
URI: https://hdl.handle.net/10356/86614
http://hdl.handle.net/10220/44156
ISSN: 2045-2322
DOI: 10.1038/s41598-017-09886-8
Rights: © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCELSE Journal Articles

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