Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86660
Title: Chikungunya virus nsP4 RNA-dependent RNA polymerase core domain displays detergent-sensitive primer extension and terminal adenylyltransferase activities
Authors: Chen, Ming Wei
Tan, Yaw Bia
Zheng, Jie
Zhao, Yongqian
Lim, Bee Ting
Cornvik, Tobias
Lescar, Julien
Ng, Lisa Fong Poh
Luo, Dahai
Keywords: Chikungunya
RdRP
Issue Date: 2017
Source: Chen, M. W., Tan, Y. B., Zheng, J., Zhao, Y., Lim, B. T., Cornvik, T., et al. (2017). Chikungunya virus nsP4 RNA-dependent RNA polymerase core domain displays detergent-sensitive primer extension and terminal adenylyltransferase activities. Antiviral Research, 143, 38-47.
Series/Report no.: Antiviral Research
Abstract: Chikungunya virus (CHIKV) is an important arboviral infectious agent in tropical and subtropical regions, often causing persistent and debilitating disease. The viral enzyme non-structural protein 4 (nsP4), as RNA-dependent RNA polymerase (RdRP), catalyzes the formation of negative-sense, genomic and subgenomic viral RNAs. Here we report a truncated nsP4 construct that is soluble, stable and purified recombinantly from Escherichia coli. Sequence analyses and homology modelling indicate that all necessary RdRP elements are included. Hydrogen/deuterium exchange with mass spectrometry was used to analyze solvent accessibility and flexibility of subdomains. Fluorophore-conjugated RNA ligands were designed and screened by using fluorescence anisotropy to select a suitable substrate for RdRP assays. Assay trials revealed that nsP4 core domain is conditionally active upon choice of detergent species, and carries out both primed extension and terminal adenylyltransferase activities. The polymerization assay can be further developed to screen for antiviral compounds in vitro.
URI: https://hdl.handle.net/10356/86660
http://hdl.handle.net/10220/44135
ISSN: 0166-3542
DOI: 10.1016/j.antiviral.2017.04.001
Rights: © 2017 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Antiviral Research, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.antiviral.2017.04.001].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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