Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/86687
Title: Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium
Authors: Pan, Chaozhi
Tan, Giin-Yu Amy
Ge, Liya
Chen, Chia-Lung
Wang, Jing-Yuan
Keywords: Glycerol Anaerobic Digestion Effluent
Polyhydroxyalkanoates
Issue Date: 2016
Source: Pan, C., Tan, G.-Y. A., Ge, L., Chen, C.-L., & Wang, J.-Y. (2016). Microbial removal of carboxylic acids from 1,3-propanediol in glycerol anaerobic digestion effluent by PHAs-producing consortium. Biochemical Engineering Journal, 112, 269-276.
Series/Report no.: Biochemical Engineering Journal
Abstract: Anaerobic fermentation of glycerol to 1,3-propanediol (1,3-PDO) is conceived as an economic feasible pathway to handle with increasing crude glycerol from biodiesel industry. However, glycerol anaerobic digestion effluent (ADE) consists of carboxylic acids and 1,3-PDO, imposing difficulties for separation. The objective of this study was, therefore, to investigate microbial removal of carboxylic acids from 1,3-PDO in glycerol ADE by polyhydroxyalkanoates (PHAs) producing consortium. Growth tests on carbon sources showed Corynebacterium hydrocarbooxydans had preference for butyrate while Bacillus megaterium for acetate and glycerol. Consequently, their consortium had a higher cell density and a faster substrate utilization rate than single strain grown in glycerol ADE. Acidic pH at 6.0 and 5.2 strongly inhibited cell growth and activity, while C:N ratio (w/w) at 8:1 could balance nitrogen demand for cell growth and PHA synthesis. Kinetic study further revealed over 80% of fed 1,3-PDO was preserved after depletion of carboxylic acids. Correspondingly, total organic carbon (TOC) contribution from 1,3-PDO rose from initial 55.8% to 84%. Produced PHAs comprised 3-hydroxybutyrate (3-HB) units. The results showed this study as the first attempt to provide a win-win solution to remove carboxylic acids from 1,3-PDO in glycerol ADE and converted them into PHAs as a secondary value-added product.
URI: https://hdl.handle.net/10356/86687
http://hdl.handle.net/10220/44151
ISSN: 1369-703X
DOI: 10.1016/j.bej.2016.04.031
Rights: © 2016 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Biochemical Engineering Journal, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1016/j.bej.2016.04.031].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:CEE Journal Articles

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