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Title: Buffalo nasal odorant-binding protein (bunOBP) and its structural evaluation with putative pheromones
Authors: Muthukumar, Subramanian
Rajesh, Durairaj
Selvam, Ramu Muthu
Saibaba, Ganesan
Suvaithenamudhan, Suvaiyarasan
Padmanabhan, Parasuraman
Gulyas, Balazs
Archunan, Govindaraju
Mohammad Abdulkader Akbarsha
Keywords: Odorant-binding Protein (OBP)
Putative Pheromones
Issue Date: 2018
Source: Muthukumar, S., Rajesh, D., Selvam, R. M., Saibaba, G., Suvaithenamudhan, S., Akbarsha, M. A., et al. (2018). Buffalo nasal odorant-binding protein (bunOBP) and its structural evaluation with putative pheromones. Scientific Reports, 8(1), 9323-.
Series/Report no.: Scientific Reports
Abstract: Pheromones are odoriferous volatile chemical cues produced by animals for communication among conspecifics so as to regulate their social behaviors. In general, the odor compounds are recognized by receptors in the nasal cavity. Odorant-binding protein (OBP), a lipocalin family protein, mediates the air-borne odor cues to nasal receptors through nasal mucus. The presence of OBP in several mammalian species is well documented but to-date there is no report of a nasal OBP in buffalo. Hence, the present study was undertaken to investigate if OBP is present in buffalo nasal mucus. Uni- and two-dimensional gel electrophoresis of the nasal mucus suggested the presence of OBP, which was confirmed using mass spectrometry. In silico homology model of the OBP was generated and its structural similarity with other mammalian OBPs was assessed. Finally, molecular-docking and -dynamics simulations analysis revealed the efficiency of buffalo nasal OBP (bunOBP) to bind with buffalo pheromones as well as other reported chemical cues. Taken together, the occurrence of nasal OBP in buffalo and its putative role in odor binding are reported for the first time. The potential association of this protein with estrus-specific volatiles could be taken to advantage for non-invasive detection of estrus in buffaloes.
ISSN: 2045-2322
DOI: 10.1038/s41598-018-27550-7
Rights: © 2018 The Author(s) (Nature Publishing Group). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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