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Title: Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography
Authors: Chen, Si
Liu, Xinyu
Wang, Nanshuo
Wang, Xianghong
Xiong, Qiaozhou
Bo, En
Yu, Xiaojun
Chen, Shufen
Liu, Linbo
Keywords: Corneal
Issue Date: 2017
Source: Chen, S., Liu, X., Wang, N., Wang, X., Xiong, Q., Bo, E., et al. (2017). Visualizing Micro-anatomical Structures of the Posterior Cornea with Micro-optical Coherence Tomography. Scientific Reports, 7, 10752-.
Series/Report no.: Scientific Reports
Abstract: Diagnosis of corneal disease and challenges in corneal transplantation require comprehensive understanding of corneal anatomy, particularly that of the posterior cornea. Micro-optical coherence tomography (µOCT) is a potentially suitable tool to meet this need, owing to its ultrahigh isotropic spatial resolution, high image acquisition rate and depth priority scanning mode. In this study, we explored the ability of µOCT to visualize micro-anatomical structures of the posterior cornea ex vivo and in vivo using small and large animals. µOCT clearly delineated cornea layers and revealed micro-anatomical structures, including not only polygonal endothelial cells, stellate keratocytes, collagen fibres and corneal nerve fibres but also new structures such as the dome-shaped basolateral side of endothelial cells and lattice structures at the interface between endothelium and Descemet’s membrane. Based on these observations, a short post-harvest longitudinal study was conducted on rat cornea to test the feasibility of using µOCT to monitor the quality of endothelial cells. This study successfully reveals a series of morphological features and pathological changes in the posterior cornea at the cellular level in situ and in real time with µOCT. These findings enrich knowledge of corneal anatomy and suggest that µOCT may be a promising imaging tool in corneal transplantation.
ISSN: 2045-2322
DOI: 10.1038/s41598-017-11380-0
Rights: © 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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