Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87029
Title: De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis
Authors: Ding, Chunming
Lim, Yen Ching
Chia, Sook Yoong
Walet, Arcinas Camille Esther
Xu, Shaohai
Lo, Alice Kinyui
Zhao, Yanling
Zhu, Dewen
Shan, Zhihui
Chen, Qingfeng
Leow, Melvin Khee-Shing
Xu, Dan
Sun, Lei
Keywords: Adipose
Brown Adipogenesis
Issue Date: 2018
Source: Ding, C., Lim, Y. C., Chia, S. Y., Walet, A. C. E., Xu, S., Lo, A. K., et al. (2018). De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis. Nature Communications, 9(1), 1329-.
Series/Report no.: Nature Communications
Abstract: Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs. A total of 169 conserved human lncRNAs show positive correlation with their nearby mRNAs, and knockdown assay supports a role of lncRNAs in regulating their nearby mRNAs. The knockdown of one of those, lnc-dPrdm16, impairs brown adipocyte differentiation in vitro and a significant reduction of BAT-selective markers in in vivo. Together, our work provides a comprehensive human adipose catalog built from diverse fat depots and establishes a roadmap to facilitate the discovery of functional lncRNAs in adipocyte development.
URI: https://hdl.handle.net/10356/87029
http://hdl.handle.net/10220/45300
DOI: 10.1038/s41467-018-03754-3
Rights: © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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