Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87043
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBose, Krishnashishen
dc.contributor.authorLech, Christopher Jacquesen
dc.contributor.authorHeddi, Brahimen
dc.contributor.authorPhan, Anh Tuânen
dc.date.accessioned2018-07-27T03:42:08Zen
dc.date.accessioned2019-12-06T16:33:53Z-
dc.date.available2018-07-27T03:42:08Zen
dc.date.available2019-12-06T16:33:53Z-
dc.date.issued2018en
dc.identifier.citationBose, K., Lech, C. J., Heddi, B., & Phan, A. T. (2018). High-resolution AFM structure of DNA G-wires in aqueous solution. Nature Communications, 9(1), 1959-.en
dc.identifier.urihttps://hdl.handle.net/10356/87043-
dc.description.abstractWe investigate the self-assembly of short pieces of the Tetrahymena telomeric DNA sequence d[G4T2G4] in physiologically relevant aqueous solution using atomic force microscopy (AFM). Wire-like structures (G-wires) of 3.0 nm height with well-defined surface periodic features were observed. Analysis of high-resolution AFM images allowed their classification based on the periodicity of these features. A major species is identified with periodic features of 4.3 nm displaying left-handed ridges or zigzag features on the molecular surface. A minor species shows primarily left-handed periodic features of 2.2 nm. In addition to 4.3 and 2.2 nm ridges, background features with periodicity of 0.9 nm are also observed. Using molecular modeling and simulation, we identify a molecular structure that can explain both the periodicity and handedness of the major G-wire species. Our results demonstrate the potential structural diversity of G-wire formation and provide valuable insight into the structure of higher-order intermolecular G-quadruplexes. Our results also demonstrate how AFM can be combined with simulation to gain insight into biomolecular structure.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent9 p.en
dc.language.isoenen
dc.relation.ispartofseriesNature Communicationsen
dc.rights© 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectAtomic Force Microscopyen
dc.subjectDNAen
dc.titleHigh-resolution AFM structure of DNA G-wires in aqueous solutionen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen
dc.identifier.doi10.1038/s41467-018-04016-yen
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:SPMS Journal Articles
Files in This Item:
File Description SizeFormat 
High-resolution AFM structure of DNA G-wires in aqueous solution.pdf4.39 MBAdobe PDFThumbnail
View/Open

SCOPUSTM   
Citations 10

54
Updated on Mar 27, 2024

Web of ScienceTM
Citations 10

44
Updated on Oct 29, 2023

Page view(s) 50

450
Updated on Mar 29, 2024

Download(s) 50

104
Updated on Mar 29, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.