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Title: Endomicroscopic optical coherence tomography for cellular resolution imaging of gastrointestinal tracts
Authors: Luo, Yuemei
Cui, Dongyao
Yu, Xiaojun
Bo, En
Wang, Xianghong
Wang, Nanshuo
Braganza, Cilwyn S.
Chen, Shufen
Liu, Xinyu
Xiong, Qiaozhou
Chen, Si
Chen, Shi
Liu, Linbo
Keywords: Optical Coherence Tomography
Endoscopic Imaging
Issue Date: 2017
Source: Luo, Y., Cui, D., Yu, X., Bo, E., Wang, X., Wang, N., et al. (2018). Endomicroscopic optical coherence tomography for cellular resolution imaging of gastrointestinal tracts. Journal of Biophotonics, in press.
Series/Report no.: Journal of Biophotonics
Abstract: Our ability to detect neoplastic changes in gastrointestinal (GI) tracts is limited by the lack of an endomicroscopic imaging tool that provides cellular-level structural details of GI mucosa over a large tissue area. In this article, we report a fiber-optic-based micro-optical coherence tomography (μOCT) system and demonstrate its capability to acquire cellular-level details of GI tissue through circumferential scanning. The system achieves an axial resolution of 2.48 μm in air and a transverse resolution of 4.8 μm with a depth-of-focus (DOF) of ~150 μm. To mitigate the issue of limited DOF, we used a rigid sheath to maintain a circular lumen and center the distal-end optics. The sensitivity is tested to be 98.8 dB with an illumination power of 15.6 mW on the sample. With fresh swine colon tissues imaged ex vivo, detailed structures such as crypt lumens and goblet cells can be clearly resolved, demonstrating that this fiber-optic μOCT system is capable of visualizing cellular-level morphological features. We also demonstrate that time-lapsed frame averaging and imaging speckle reduction are essential for clearly visualizing cellular-level details. Further development of a clinically viable μOCT endomicroscope is likely to improve the diagnostic outcome of GI cancers.
ISSN: 1864-063X
DOI: 10.1002/jbio.201700141
Schools: School of Chemical and Biomedical Engineering 
School of Electrical and Electronic Engineering 
Rights: © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. This is the author created version of a work that has been peer reviewed and accepted for publication by Journal of Biophotonics, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [].
Fulltext Permission: open
Fulltext Availability: With Fulltext
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