Please use this identifier to cite or link to this item:
Title: A conserved mechanism for binding of p53 DNA-Binding domain and Anti-Apoptotic Bcl-2 family proteins
Authors: Lee, Dong-Hwa
Ha, Ji-Hyang
Kim, Yul
Jang, Mi
Park, Sung Jean
Yoon, Ho Sup
Kim, Eun-Hee
Bae, Kwang-Hee
Park, Byoung Chul
Park, Sung Goo
Yi, Gwan-Su
Chi, Seung-Wook
Keywords: DRNTU::Science::Biological sciences
Bcl-2 Family Proteins
Issue Date: 2014
Source: Lee, D.-H., Ha, J.-H., Kim, Y., Jang, M., Park, S. J., Yoon, H. S., . . . Chi, S.-W. (2014). A Conserved Mechanism for Binding of p53 DNA-Binding Domain and Anti-Apoptotic Bcl-2 Family Proteins. Molecules and Cells, 37(3), 264-269. doi:10.14348/molcells.2014.0001
Series/Report no.: Molecules and Cells
Abstract: The molecular interaction between tumor suppressor p53 and the anti-apoptotic Bcl-2 family proteins plays an essential role in the transcription-independent apoptotic pathway of p53. In this study, we investigated the binding of p53 DNA-binding domain (p53DBD) with the anti-apoptotic Bcl-2 family proteins, Bcl-w, Mcl-1, and Bcl-2, using GST pull-down assay and NMR spectroscopy. The GST pull-down assays and NMR experiments demonstrated the direct binding of the p53DBD with Bcl-w, Mcl-1, and Bcl-2. Further, NMR chemical shift perturbation data showed that Bcl-w and Mcl-1 bind to the positively charged DNA-binding surface of p53DBD. Noticeably, the refined structural models of the complexes between p53DBD and Bcl-w, Mcl-1, and Bcl-2 showed that the binding mode of p53DBD is highly conserved among the anti-apoptotic Bcl-2 family proteins. Furthermore, the chemical shift perturbations on Bcl-w, Mcl-1, and Bcl-2 induced by p53DBD binding occurred not only at the p53DBD-binding acidic region but also at the BH3 peptide-binding pocket, which suggests an allosteric conformational change similar to that observed in Bcl-XL. Taken altogether, our results revealed a structural basis for a conserved binding mechanism between p53DBD and the anti-apoptotic Bcl-2 family proteins, which shed light on to the molecular understanding of the transcription-independent apoptosis pathway of p53.
ISSN: 1016-8478
DOI: 10.14348/molcells.2014.0001
Schools: School of Biological Sciences 
Rights: © 2014 The Korean Society for Molecular and Cellular Biology. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

Citations 20

Updated on Feb 22, 2024

Web of ScienceTM
Citations 20

Updated on Oct 29, 2023

Page view(s) 50

Updated on Feb 24, 2024

Download(s) 50

Updated on Feb 24, 2024

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.