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dc.contributor.authorChen, Feien
dc.contributor.authorChen, Gukuien
dc.contributor.authorLiu, Yiweien
dc.contributor.authorJin, Yongxinen
dc.contributor.authorCheng, Zhihuien
dc.contributor.authorLiu, Yangen
dc.contributor.authorYang, Liangen
dc.contributor.authorJin, Shouguangen
dc.contributor.authorWu, Weihuien
dc.identifier.citationChen, F., Chen, G., Liu, Y., Jin, Y., Cheng, Z., Liu, Y., et al. (2017). Pseudomonas aeruginosa Oligoribonuclease Contributes to Tolerance to Ciprofloxacin by Regulating Pyocin Biosynthesis. Antimicrobial Agents and Chemotherapy, 61(3), e02256-16-.en
dc.description.abstractBacterial oligoribonuclease (Orn) is a conserved 3′-to-5′ exonuclease. In Pseudomonas aeruginosa, it has been demonstrated that Orn plays a major role in the hydrolysis of pGpG, which is required for cyclic-di-GMP homeostasis. Meanwhile, Orn is involved in the degradation of nanoRNAs, which can alter global gene expression by serving as transcription initiation primers. Previously, we found that Orn is required for the type III secretion system and pathogenesis of P. aeruginosa, indicating a role of Orn in the bacterial response to environmental stimuli. Here we report that Orn is required for the tolerance of P. aeruginosa to ciprofloxacin. Transcriptome analysis of an orn mutant revealed the upregulation of pyocin biosynthesis genes. Mutation of genes involved in pyocin biosynthesis in the background of an orn mutant restored bacterial tolerance to ciprofloxacin. We further demonstrate that the upregulation of pyocin biosynthesis genes is due to RecA-mediated autoproteolysis of PrtR, which is the major negative regulator of pyocin biosynthesis genes. In addition, the SOS response genes were upregulated in the orn mutant, indicating a DNA damage stress. Therefore, our results revealed a novel role of Orn in bacterial tolerance to ciprofloxacin.en
dc.format.extent11 p.en
dc.relation.ispartofseriesAntimicrobial Agents and Chemotherapyen
dc.rights© 2017 American Society for Microbiology. This paper was published in Antimicrobial Agents and Chemotherapy and is made available as an electronic reprint (preprint) with permission of American Society for Microbiology. The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.titlePseudomonas Aeruginosa Oligoribonuclease contributes to tolerance to ciprofloxacin by regulating pyocin biosynthesisen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.description.versionPublished versionen
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