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Title: Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles
Authors: Sze, Siu Kwan
van de Weg, Sander M.
Vernooij, Flora
Schoneveld, Arjan
Tan, Sock-Hwee
Versteeg, Henri
Timmers, Leo
Lam, Carolyn
Wang, Jiong-Wei
Zhang, Ya-Nan
de Kleijn, Dominique P.V.
Keywords: Extracellular Vesicles
Lipoprotein Particles
Issue Date: 2018
Source: Wang, J.-W., Zhang, Y.-N., Sze, S. K., van de Weg, S. M., Vernooij, F., Schoneveld, A., et al. (2018). Lowering Low-Density Lipoprotein Particles in Plasma Using Dextran Sulphate Co-Precipitates Procoagulant Extracellular Vesicles. International Journal of Molecular Sciences, 19(1), 94-.
Series/Report no.: International Journal of Molecular Sciences
Abstract: Plasma extracellular vesicles (EVs) are lipid membrane vesicles involved in several biological processes including coagulation. Both coagulation and lipid metabolism are strongly associated with cardiovascular events. Lowering very-low- and low-density lipoprotein ((V)LDL) particles via dextran sulphate LDL apheresis also removes coagulation proteins. It remains unknown, however, how coagulation proteins are removed in apheresis. We hypothesize that plasma EVs that contain high levels of coagulation proteins are concomitantly removed with (V)LDL particles by dextran sulphate apheresis. For this, we precipitated (V)LDL particles from human plasma with dextran sulphate and analyzed the abundance of coagulation proteins and EVs in the precipitate. Coagulation pathway proteins, as demonstrated by proteomics and a bead-based immunoassay, were over-represented in the (V)LDL precipitate. In this precipitate, both bilayer EVs and monolayer (V)LDL particles were observed by electron microscopy. Separation of EVs from (V)LDL particles using density gradient centrifugation revealed that almost all coagulation proteins were present in the EVs and not in the (V)LDL particles. These EVs also showed a strong procoagulant activity. Our study suggests that dextran sulphate used in LDL apheresis may remove procoagulant EVs concomitantly with (V)LDL particles, leading to a loss of coagulation proteins from the blood.
ISSN: 1661-6596
DOI: 10.3390/ijms19010094
Rights: © 2017 by The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
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