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dc.contributor.authorHoang, Kim Le Maien
dc.contributor.authorTran, Hoa Thien
dc.contributor.authorLow, Zhen Jieen
dc.contributor.authorPang, Li Meien
dc.contributor.authorDing, Yichenen
dc.contributor.authorCheang, Qing Weien
dc.contributor.authorLi, Jinmingen
dc.contributor.authorLiu, Xue-Weien
dc.contributor.authorKanagasundaram, Yoganathanen
dc.contributor.authorYang, Liangen
dc.contributor.authorLiang, Zhao-Xunen
dc.identifier.citationLow, Z. J., Pang, L. M., Ding, Y., Cheang, Q. W., Hoang, K. L. M., Tran, H. T., et al. (2018). Identification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sediment. Scientific Reports, 8(1), 1594-.en
dc.description.abstractStreptomyces are a genus of Actinobacteria capable of producing structurally diverse natural products. Here we report the isolation and characterization of a biosynthetically talented Streptomyces (Streptomyces sp. SD85) from tropical mangrove sediments. Whole-genome sequencing revealed that Streptomyces sp. SD85 harbors at least 52 biosynthetic gene clusters (BGCs), which constitute 21.2% of the 8.6-Mb genome. When cultivated under lab conditions, Streptomyces sp. SD85 produces sceliphrolactam, a 26-membered polyene macrolactam with unknown biosynthetic origin. Genome mining yielded a putative sceliphrolactam BGC (sce) that encodes a type I modular polyketide synthase (PKS) system, several β-amino acid starter biosynthetic enzymes, transporters, and transcriptional regulators. Using the CRISPR/Cas9–based gene knockout method, we demonstrated that the sce BGC is essential for sceliphrolactam biosynthesis. Unexpectedly, the PKS system encoded by sce is short of one module required for assembling the 26-membered macrolactam skeleton according to the collinearity rule. With experimental data disfavoring the involvement of a trans-PKS module, the biosynthesis of sceliphrolactam seems to be best rationalized by invoking a mechanism whereby the PKS system employs an iterative module to catalyze two successive chain extensions with different outcomes. The potential violation of the collinearity rule makes the mechanism distinct from those of other polyene macrolactams.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent13 p.en
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2018 The Author(s) (Nature Publishing Group). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
dc.subjectPolyene Macrolactam Sceliphrolactamen
dc.titleIdentification of a biosynthetic gene cluster for the polyene macrolactam sceliphrolactam in a Streptomyces strain isolated from mangrove sedimenten
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.description.versionPublished versionen
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