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Title: Thrombin and plasmin alter the proteome of neutrophil extracellular traps
Authors: Lim, Chun Hwee
Adav, Sunil Shankar
Sze, Siu Kwan
Choong, Yeu Khai
Saravanan, Rathi
Schmidtchen, Artur
Keywords: Neutrophil Extracellular Traps
Issue Date: 2018
Source: Lim, C. H., Adav, S. S., Sze, S. K., Choong, Y. K., Saravanan, R., & Schmidtchen, A. (2018). Thrombin and plasmin alter the proteome of neutrophil extracellular traps, 9, 1554-.
Series/Report no.: Frontiers in Immunology
Abstract: Neutrophil extracellular traps (NETs) consist of a decondensed DNA scaffold decorated with neutrophil-derived proteins. The proteome of NETs, or “NETome,” has been largely elucidated in vitro. However, components such as plasma and extracellular matrix proteins may affect the NETome under physiological conditions. Here, using a reductionistic approach, we explored the effects of two proteases active during injury and wounding, human thrombin and plasmin, on the NETome. Using high-resolution mass spectrometry, we identified a total of 164 proteins, including those previously not described in NETs. The serine proteases, particularly thrombin, were also found to interact with DNA and bound to NETs in vitro. Among the most abundant proteins were those identified previously, including histones, neutrophil elastase, and antimicrobial proteins. We observed reduced histone (H2B, H3, and H4) and neutrophil elastase levels upon the addition of the two proteases. Analyses of NET-derived tryptic peptides identified subtle changes upon protease treatments. Our results provide evidence that exogenous proteases, present during wounding and inflammation, influence the NETome. Taken together, regulation of NETs and their proteins under different physiological conditions may affect their roles in infection, inflammation, and the host response.
DOI: 10.3389/fimmu.2018.01554
Rights: © 2018 Lim, Adav, Sze, Choong, Saravanan and Schmidtchen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:IGS Journal Articles

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