Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/87852
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dc.contributor.authorLiu, Wenshanen
dc.contributor.authorZhang, Boen
dc.contributor.authorJiang, Rongrongen
dc.date.accessioned2018-12-07T08:54:56Zen
dc.date.accessioned2019-12-06T16:50:47Z-
dc.date.available2018-12-07T08:54:56Zen
dc.date.available2019-12-06T16:50:47Z-
dc.date.issued2017en
dc.identifier.citationLiu, W., Zhang, B., & Jiang, R. (2017). Improving acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypass. Biotechnology for Biofuels, 10, 41-. doi:10.1186/s13068-017-0726-zen
dc.identifier.issn1754-6834en
dc.identifier.urihttps://hdl.handle.net/10356/87852-
dc.description.abstractBackground: Acetyl-CoA is an important precursor in Saccharomyces cerevisiae. Various approaches have been adopted to improve its cytosolic level previously with the emphasis on engineering the “acetyl-” part of acetyl-CoA. To the best of our knowledge, there have been no reports on engineering the “-CoA” part so far. Results: In this study, we had tried to engineer S. cerevisiae from both the “-CoA” part via pantothenate kinase overexpression [PanK from S. cerevisiae, the rate-limiting enzyme for CoA synthesis] and the “acetyl-“part through PDH bypass introduction (ALD6 from S. cerevisiae and SeAcs L641P from Salmonella enteric). A naringenin-producing reporter strain had been constructed to reflect cytosolic acetyl-CoA level as acetyl-CoA is the precursor of naringenin. It was found that PanK overexpression or PDH bypass introduction alone only led to a twofold or 6.74-fold increase in naringenin titer, but the combination of both (strain CENFPAA01) had resulted in 24.4-fold increase as compared to the control (strain CENF09) in the presence of 0.5 mM substrate p-coumaric acid. The supplement of PanK substrate pantothenate resulted in another 19% increase in naringenin production. Conclusions: To greatly enhance acetyl-CoA level in yeast cytosol, it is feasible to engineer both the “acetyl-” part and the “-CoA” part simultaneously. Insufficient CoA supply might aggravate acetyl-CoA shortage and cause low yield of target product.en
dc.format.extent9 p.en
dc.language.isoenen
dc.relation.ispartofseriesBiotechnology for Biofuelsen
dc.rights© 2017 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectPantothenate Kinaseen
dc.subjectDRNTU::Engineering::Bioengineeringen
dc.subjectAcetyl-CoAen
dc.titleImproving acetyl-CoA biosynthesis in Saccharomyces cerevisiae via the overexpression of pantothenate kinase and PDH bypassen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.identifier.doi10.1186/s13068-017-0726-zen
dc.description.versionPublished versionen
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