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Title: Novel application of in vivo micro-optical coherence tomography to assess cornea scarring in an animal model
Authors: Ang, Marcus
Devarajan, Kavya
Das, Suchandrima
Yam, Gary H. F.
Htoon, Hla Mynt
Chen, Si
Liu, Xinyu
Liu, Linbo
Girard, Michael
Mehta, Jodhbir S.
Keywords: Cornea Scarring
DRNTU::Engineering::Electrical and electronic engineering
Micro-Optical Coherence Tomography (micro-OCT)
Issue Date: 2018
Source: Ang, M., Devarajan, K., Das, S., Yam, G. H. F., Htoon, H. M., Chen, S., . . . Mehta, J. S. (2018). Novel application of In Vivo Micro-Optical Coherence Tomography to assess Cornea scarring in an Animal Model. Scientific Reports, 8, 11483-. doi:10.1038/s41598-018-29761-4
Series/Report no.: Scientific Reports
Abstract: This pilot study uses a micro-optical coherence tomography (micro-OCT) system with ~1 μm axial resolution specifically to image the cornea and corneal scars in vivo. We used an established murine corneal scar model by irregular phototherapeutic keratectomy in ten C57BL/6 mice, with serial imaging using the micro-OCT and compared to anterior segment (AS-OCT) (RTvue, Optovue, Fremont, CA) before and after scar induction. Main outcome was agreement between the AS-OCT and micro-OCT using Bland-Altman plots (95% limits of agreement, LoA).We analysed 10 control eyes and 10 eyes with corneal scars and found that there was good agreement between AS-OCT and micro-OCT (P > 0.05) LOA: lower limit −14 µm (95% CI: −19 to −8.8 µm) upper limit 23 µm (95% CI: 18 to 28.5 µm) in terms of central corneal thickness. There was also good agreement between AS-OCT and micro-OCT in terms of corneal scar measurements (P > 0.5; correlation coefficient >0.99) LOA lower limit −2.1 µm (95% CI: −2.8 to −1.5 µm); upper limit 1.8 µm (95% CI: 1.1 to 2.4 µm). Our pilot study suggests that this novel in vivo micro-OCT imaging technique was able to measure central corneal thickness and scar thickness in agreement with current AS-OCT techniques.
DOI: 10.1038/s41598-018-29761-4
Rights: © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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