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|Title:||Ocular blood flow measurements in healthy white subjects using laser speckle flowgraphy||Authors:||Luft, Nikolaus
Wozniak, Piotr A.
Aschinger, Gerold C.
Bata, Ahmed M.
Werkmeister, René M.
Witkowska, Katarzyna J.
|Keywords:||Laser Speckle Flowgraphy
Blood Flow Velocity
|Issue Date:||2016||Source:||Luft, N., Wozniak, P. A., Aschinger, G. C., Fondi, K., Bata, A. M., Werkmeister, R. M., . . . Schmetterer, L. (2016). Ocular blood flow measurements in healthy white subjects using laser speckle flowgraphy. PLOS ONE, 11(12), e0168190-. doi:10.1371/journal.pone.0168190||Series/Report no.:||PLOS ONE||Abstract:||Purpose: To assess the feasibility and reliability of Laser Speckle Flowgraphy (LSFG) to measure ocular perfusion in a sample of healthy white subjects and to elucidate the age-dependence of the parameters obtained. Methods: This cross-sectional study included 80 eyes of 80 healthy, non-smoking white subjects of Western European descent between 19 and 79 years of age. A commercial LSFG instrument was applied to measure ocular blood flow at the optic nerve head (ONH) three successive times before and after pharmacological pupil dilation. The mean blur rate (MBR), a measure of relative blood flow velocity, was obtained for different regions of the ONH. Eight parameters of ocular perfusion derived from the pulse-waveform analysis of MBR including blowout time (BOT) and falling rate (FR) were also recorded. Results: Artifact-free LSFG images meeting the quality criteria for automated image analysis were obtainable in 93.8% without pupil dilation and in 98.8% with pharmacological pupil dilation. Measurements of MBR showed excellent repeatability with intraclass correlation coefficients ≥ 0.937 and were barely affected by pupil dilation. The majority of pulse-waveform derived variables exhibited equally high repeatability. MBR-related blood flow indices exhibited significant age dependence (p<0.001). FR (r = 0.747, p<0.001) and BOT (r = -0.714, p<0.001) most strongly correlated with age. Conclusions: LSFG represents a reliable method for the quantitative assessment of ocular blood flow in white subjects. Our data affirms that the LSFG-derived variables FR and BOT may be useful biomarkers for age-related changes in ocular perfusion.||URI:||https://hdl.handle.net/10356/88185
|DOI:||10.1371/journal.pone.0168190||Rights:||© 2016 Luft et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||LKCMedicine Journal Articles|
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