Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88280
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dc.contributor.authorTay, Vanessa Shi Yunen
dc.contributor.authorBoehm, Bernhard Ottoen
dc.contributor.authorDorajoo, Rajkumaren
dc.contributor.authorAli, Yusufen
dc.contributor.authorKang, Jonathanen
dc.contributor.authorSamydurai, Sudhagaren
dc.contributor.authorLiu, Jianjunen
dc.date.accessioned2018-08-27T02:29:42Zen
dc.date.accessioned2019-12-06T16:59:45Z-
dc.date.available2018-08-27T02:29:42Zen
dc.date.available2019-12-06T16:59:45Z-
dc.date.issued2017en
dc.identifier.citationDorajoo, R., Ali, Y., Tay, V. S. Y., Kang, J., Samydurai, S., Liu, J., & Boehm, B. O. (2017). Single-cell transcriptomics of East-Asian pancreatic islets cells. Scientific Reports, 7, 5024-. doi:10.1038/s41598-017-05266-4en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/10356/88280-
dc.description.abstractSingle-cell RNA-seq (scRNA-seq) of pancreatic islets have reported on α- and β-cell gene expression in mice and subjects of predominantly European ancestry. We aimed to assess these findings in East-Asian islet-cells. 448 islet-cells were captured from three East-Asian non-diabetic subjects for scRNA-seq. Hierarchical clustering using pancreatic cell lineage genes was used to assign cells into cell-types. Differentially expressed transcripts between α- and β-cells were detected using ANOVA and in silico replications of mouse and human islet cell genes were performed. We identified 118 α, 105 β, 6 δ endocrine cells and 47 exocrine cells. Besides INS and GCG, 26 genes showed differential expression between α- and β-cells. 10 genes showed concordant expression as reported in rodents, while FAM46A was significantly discordant. Comparing our East-Asian data with data from primarily European subjects, we replicated several genes implicated in nuclear receptor activations, acute phase response pathway, glutaryl-CoA/tryptophan degradations and EIF2/AMPK/mTOR signaling. Additionally, we identified protein ubiquitination to be associated among East-Asian β-cells. We report on East-Asian α- and β-cell gene signatures and substantiate several genes/pathways. We identify expression signatures in East-Asian β-cells that perhaps reflects increased susceptibility to cell-death and warrants future validations to fully appreciate their role in East-Asian diabetes pathogenesis.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent8 p.en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectGene Expressionen
dc.subjectIslet Cellen
dc.subjectDRNTU::Science::Biological sciencesen
dc.titleSingle-cell transcriptomics of East-Asian pancreatic islets cellsen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.identifier.doi10.1038/s41598-017-05266-4en
dc.description.versionPublished versionen
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