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Title: Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis
Authors: Cheng, Penghui
Zhang, Jianjian
Huang, Jiaguo
Miao, Qingqing
Xu, Chenjie
Pu, Kanyi
Keywords: Cell Culture
DRNTU::Engineering::Chemical engineering
Issue Date: 2018
Source: Cheng, P., Zhang, J., Huang, J., Miao, Q., Xu, C., & Pu, K. (2018). Near-infrared fluorescence probes to detect reactive oxygen species for keloid diagnosis. Chemical Science, 9(30), 6340-6347. doi:10.1039/c8sc01865k
Series/Report no.: Chemical Science
Abstract: Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids – a benign dermal tumour developed after lesion, the ability of employing RONS probes for keloid detection has not yet been exploited. Herein, we report two near-infrared (NIR) fluorescent probes (CyTF and CyBA) that can specifically distinguish keloid fibroblasts from normal dermal fibroblasts. Both CyTF and CyBA show a 15-fold NIR fluorescence enhancement at 717 nm upon reaction with RONS. However, because CyTF has higher specificity towards ONOO− than CyBA, CyTF can detect stimulated fibroblasts in a more sensitive way, showing 3.76 and 2.26-fold fluorescence increments in TGF-β1 stimulated dermal fibroblasts and keloid fibroblasts, respectively. Furthermore, CyTF permits specific detection of implanted keloid fibroblasts in a xenograft live mouse model. Our work thus developed a new optical imaging approach that has the potential for early diagnosis and drug screening of keloids.
ISSN: 2041-6520
DOI: 10.1039/C8SC01865K
Schools: School of Chemical and Biomedical Engineering 
Rights: © 2018 The Royal Society of Chemistry. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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