Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88465
Title: A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
Authors: Hou, Aihua
Bose, Tanima
Chandy, Kanianthara George
Tong, Louis
Keywords: Immunology
Dry Eye Disease
Issue Date: 2017
Source: Hou, A., Bose, T., Chandy, K. G., & Tong, L. (2017). A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue. Journal of Visualized Experiments, (124), e55592-.
Series/Report no.: Journal of Visualized Experiments
Abstract: Dry eye disease is a very common condition that causes morbidity and healthcare burden and decreases the quality of life. There is a need for a suitable dry eye animal model to test novel therapeutics to treat autoimmune dry eye conditions. This protocol describes a chronic autoimmune dry eye rat model. Lewis rats were immunized with an emulsion containing lacrimal gland extract, ovalbumin, and complete Freund's adjuvant. A second immunization with the same antigens in incomplete Freund's adjuvant was administered two weeks later. These immunizations were administered subcutaneously at the base of the tail. To boost the immune response at the ocular surface and lacrimal glands, lacrimal gland extract and ovalbumin were injected into the forniceal subconjunctiva and lacrimal glands 6 weeks after the first immunization. The rats developed dry eye features, including reduced tear production, decreased tear stability, and increased corneal damage. Immune profiling by flow cytometry showed a preponderance of CD3+ effector memory T cells in the eyeball.
URI: https://hdl.handle.net/10356/88465
http://hdl.handle.net/10220/44618
DOI: 10.3791/55592
Rights: © 2017 The Author(s) (Journal of Visualized Experiments). This paper was published under Creative Commons Attribution-Non Commercial-No Derivs 3.0 Unported Agreement in Journal of Visualized Experiments and is made available as an electronic reprint (preprint) with permission of The Author(s) (Journal of Visualized Experiments). The published version is available at: [http://dx.doi.org/10.3791/55592]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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