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Title: ANRIL promoter DNA methylation : a perinatal marker for later adiposity
Authors: Lillycrop, Karen
Murray, Robert
Cheong, Clara
Teh, Ai Ling
Clarke-Harris, Rebecca
Barton, Sheila
Costello, Paula
Garratt, Emma
Cook, Eloise
Titcombe, Philip
Shunmuganathan, Bhuvaneshwari
Liew, Samantha J.
Chua, Yong-Cai
Lin, Xinyi
Wu, Yonghui
Burdge, Graham C.
Cooper, Cyrus
Inskip, Hazel M.
Karnani, Neerja
Hopkins, James C.
Childs, Caroline E.
Chavez, Carolina Paras
Calder, Philip C.
Yap, Fabian
Lee, Yung Seng
Chong, Yap Seng
Melton, Philip E.
Beilin, Lawrie
Huang, Rae-Chi
Gluckman, Peter D.
Harvey, Nick
Hanson, Mark A.
Holbrook, Joanna D.
Godfrey, Keith M.
Keywords: Adiposity
DNA Methylation
Issue Date: 2017
Source: Lillycrop, K., Murray, R., Cheong, C., Teh, A. L., Clarke-Harris, R., Barton, S., . . . Godfrey, K. M. (2017). ANRIL Promoter DNA Methylation: A Perinatal Marker for Later Adiposity. EBioMedicine, 19, 60-72. doi:10.1016/j.ebiom.2017.03.037
Series/Report no.: EBioMedicine
Abstract: Experimental studies show a substantial contribution of early life environment to obesity risk through epigenetic processes. We examined inter-individual DNA methylation differences in human birth tissues associated with child's adiposity. We identified a novel association between the level of CpG methylation at birth within the promoter of the long non-coding RNA ANRIL (encoded at CDKN2A) and childhood adiposity at age 6-years. An association between ANRIL methylation and adiposity was also observed in three additional populations; in birth tissues from ethnically diverse neonates, in peripheral blood from adolescents, and in adipose tissue from adults. Additionally, CpG methylation was associated with ANRIL expression in vivo, and CpG mutagenesis in vitro inhibited ANRIL promoter activity. Furthermore, CpG methylation enhanced binding to an Estrogen Response Element within the ANRIL promoter. Our findings demonstrate that perinatal methylation at loci relevant to gene function may be a robust marker of later adiposity, providing substantial support for epigenetic processes in mediating long-term consequences of early life environment on human health.
DOI: 10.1016/j.ebiom.2017.03.037
Rights: © 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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