Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88907
Title: Early developmental perturbations in a human stem cell model of MODY5/HNF1B pancreatic hypoplasia
Authors: Teo, Adrian Kee Keong
Lau, Hwee Hui
Valdez, Ivan Achel
Dirice, Ercument
Tjora, Erling
Raeder, Helge
Kulkarni, Rohit N.
Keywords: DRNTU::Science::Medicine
Stem Cell
Molecular Mechanisms
Issue Date: 2016
Source: Teo, A., Lau, H., Valdez, I., Dirice, E., Tjora, E., Raeder, H., & Kulkarni, R. (2016). Early Developmental Perturbations in a Human Stem Cell Model of MODY5/HNF1B Pancreatic Hypoplasia. Stem Cell Reports, 6(3), 357-367. doi:10.1016/j.stemcr.2016.01.007
Series/Report no.: Stem Cell Reports
Abstract: Patients with an HNF1BS148L/+ mutation (MODY5) typically exhibit pancreatic hypoplasia. However, the molecular mechanisms are unknown due to inaccessibility of patient material and because mouse models do not fully recapitulate MODY5. Here, we differentiated MODY5 human-induced pluripotent stem cells (hiPSCs) into pancreatic progenitors, and show that the HNF1BS148L/+ mutation causes a compensatory increase in several pancreatic transcription factors, and surprisingly, a decrease in PAX6 pancreatic gene expression. The lack of suppression of PDX1, PTF1A, GATA4, and GATA6 indicates that MODY5-mediated pancreatic hypoplasia is mechanistically independent. Overexpression studies demonstrate that a compensatory increase in PDX1 gene expression is due to mutant HNF1BS148L/+ but not wild-type HNF1B or HNF1A. Furthermore, HNF1B does not appear to directly regulate PAX6 gene expression necessary for glucose tolerance. Our results demonstrate compensatory mechanisms in the pancreatic transcription factor network due to mutant HNF1BS148L/+ protein. Thus, patients typically develop MODY5 but not neonatal diabetes despite exhibiting pancreatic hypoplasia.
URI: https://hdl.handle.net/10356/88907
http://hdl.handle.net/10220/46988
DOI: 10.1016/j.stemcr.2016.01.007
Rights: © 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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