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Title: Enhancement of cardiomyogenesis in stem cells by low intensity pulsed ultrasound
Authors: Teo, Ailing
Morshedi, Amir
Wang, Jen-Chieh
Lim, Mayasari
Zhou, Yufeng
Keywords: DRNTU::Engineering::Bioengineering
Stem Cells
Low Intensity Pulsed Ultrasound
Issue Date: 2017
Source: Teo, A., Morshedi, A., Wang, J.-C., Lim, M., & Zhou, Y. (2017). Enhancement of cardiomyogenesis in stem cells by low intensity pulsed ultrasound. AIP Conference Proceedings, 1821, 210002-. doi:10.1063/1.4977671
Series/Report no.: AIP Conference Proceedings
Abstract: Low intensity pulsed ultrasound (LIPUS) has been shown to enhance bone and cartilage regeneration from stem cells. Gene expression of angiotensin II type 1 (AT1) receptor can be increased in LIPUS-treated osteoblasts. The AT1 receptor is a known mechanoreceptor in cardiomyocytes. It suggests that LIPUS may enhance cardiomyogenesis via mechanotransduction by increasing AT1 expression. Murine embryonic stem cells (ESCs) were treated daily by 10-min 1MHz LIPUS at spatial-average temporal-peak acoustic intensities of 30 mW/cm2 and 300 mW/cm2 in both continuous and pulsed wave (20% duty cycle) for 10 days. Polymerase chain reaction (PCR), immunocytochemistry, and beating rate were used to evaluate the cardiac viability quantitatively. After the treatment of LIPUS, beating rate of contractile areas and cardiac gene expression, such as α- and β-myosin heavy chain, were improved. Furthermore, no deleterious effects to the development of cardiac proteins were observed. All results suggest that LIPUS stimulation has the capacity of enhancing cardiomyogenesis from embryonic stem cells. With the benefit and the ease in incorporating LIPUS into various culture platforms, LIPUS has the potential to produce cardiomyocytes for clinical use in the future.
ISSN: 0094-243X
DOI: 10.1063/1.4977671
Rights: © 2017 The Author(s) (Published by AIP). This paper was published in AIP Conference Proceedings and is made available as an electronic reprint (preprint) with permission of the author(s) (published by AIP). The published version is available at: []. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SCBE Journal Articles

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