Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/88947
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dc.contributor.authorTeo, Ailingen
dc.contributor.authorMorshedi, Amiren
dc.contributor.authorWang, Jen-Chiehen
dc.contributor.authorLim, Mayasarien
dc.contributor.authorZhou, Yufengen
dc.date.accessioned2018-09-19T04:19:51Zen
dc.date.accessioned2019-12-06T17:14:22Z-
dc.date.available2018-09-19T04:19:51Zen
dc.date.available2019-12-06T17:14:22Z-
dc.date.issued2017en
dc.identifier.citationTeo, A., Morshedi, A., Wang, J.-C., Lim, M., & Zhou, Y. (2017). Enhancement of cardiomyogenesis in stem cells by low intensity pulsed ultrasound. AIP Conference Proceedings, 1821, 210002-. doi:10.1063/1.4977671en
dc.identifier.issn0094-243Xen
dc.identifier.urihttps://hdl.handle.net/10356/88947-
dc.description.abstractLow intensity pulsed ultrasound (LIPUS) has been shown to enhance bone and cartilage regeneration from stem cells. Gene expression of angiotensin II type 1 (AT1) receptor can be increased in LIPUS-treated osteoblasts. The AT1 receptor is a known mechanoreceptor in cardiomyocytes. It suggests that LIPUS may enhance cardiomyogenesis via mechanotransduction by increasing AT1 expression. Murine embryonic stem cells (ESCs) were treated daily by 10-min 1MHz LIPUS at spatial-average temporal-peak acoustic intensities of 30 mW/cm2 and 300 mW/cm2 in both continuous and pulsed wave (20% duty cycle) for 10 days. Polymerase chain reaction (PCR), immunocytochemistry, and beating rate were used to evaluate the cardiac viability quantitatively. After the treatment of LIPUS, beating rate of contractile areas and cardiac gene expression, such as α- and β-myosin heavy chain, were improved. Furthermore, no deleterious effects to the development of cardiac proteins were observed. All results suggest that LIPUS stimulation has the capacity of enhancing cardiomyogenesis from embryonic stem cells. With the benefit and the ease in incorporating LIPUS into various culture platforms, LIPUS has the potential to produce cardiomyocytes for clinical use in the future.en
dc.format.extent5 p.en
dc.language.isoenen
dc.relation.ispartofseriesAIP Conference Proceedingsen
dc.rights© 2017 The Author(s) (Published by AIP). This paper was published in AIP Conference Proceedings and is made available as an electronic reprint (preprint) with permission of the author(s) (published by AIP). The published version is available at: [http://dx.doi.org/10.1063/1.4977671]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectDRNTU::Engineering::Bioengineeringen
dc.subjectStem Cellsen
dc.subjectLow Intensity Pulsed Ultrasounden
dc.titleEnhancement of cardiomyogenesis in stem cells by low intensity pulsed ultrasounden
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen
dc.contributor.conferenceProceedings from the 14th International Symposium on Therapeutic Ultrasounden
dc.identifier.doi10.1063/1.4977671en
dc.description.versionPublished versionen
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