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dc.contributor.authorKim, Jaejungen
dc.contributor.authorLyu, Xiao Meien
dc.contributor.authorLee, Jaslyn Jie Linen
dc.contributor.authorZhao, Guilien
dc.contributor.authorChin, Seow Fongen
dc.contributor.authorYang, Liangen
dc.contributor.authorChen, Wei Ningen
dc.identifier.citationKim, J., Lyu, X. M., Lee, J. J. L., Zhao, G., Chin, S. F., Yang, L., & Chen, W. N. (2018). Metabolomics analysis of Pseudomonas chlororaphis JK12 algicidal activity under aerobic and micro-aerobic culture condition. AMB Express, 8(1), 131-. doi:10.1186/s13568-018-0660-xen
dc.description.abstractUtilization of algicidal bacteria as a biological agent have been receiving significant interest for controlling harmful algal blooms. While various algicidal bacterial strains have been identified, limited studies have explored the influence of bacterial culture conditions on its algicidal activity. Here, the effect of oxygen on the algicidal activity of a novel bacterium JK12, against a model diatom, Phaeodactylum tricornutum (P. tricornutum) was studied. Strain JK12 showed high algicidal activity against P. tricornutum and was identified as Pseudomonas chlororaphis (P. chlororaphis) by 16S ribosomal RNA gene analysis. JK12 culture supernatant exhibited strong algicidal activity while washed JK12 cells showed no obvious activity, indicating that JK12 indirectly attacks algae by secreting extracellular algicidal metabolites. Micro-aerobic culture condition dramatically enhanced the algicidal activity of JK12 by 50%, compared to that cultured under aerobic condition in 24 h. Extracellular metabolomic profiling of JK12 using gas chromatography–mass spectrometry and liquid chromatography–mass spectrometry analysis revealed significantly higher amounts of allantoic acid, urocanic acid, cytidine 2′,3′-cyclic phosphate, uridine 2′,3′-cyclic phosphate, and chlorinated tryptophan in the micro-aerobic culture. This is the first report to demonstrate the important role of oxygen on the algicidal activity of a non-pathogenic strain P. chlororaphis. In addition, the metabolomics analysis provided insights into the algicidal mechanism of P. chlororaphis.en
dc.format.extent10 p.en
dc.relation.ispartofseriesAMB Expressen
dc.rights© 2018 The Author(s). This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.subjectAlgicidal Bacteriaen
dc.subjectPseudomonas Chlororaphisen
dc.subjectDRNTU::Engineering::Chemical engineeringen
dc.titleMetabolomics analysis of Pseudomonas chlororaphis JK12 algicidal activity under aerobic and micro-aerobic culture conditionen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.researchSingapore Centre for Environmental Life Sciences Engineeringen
dc.description.versionPublished versionen
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