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Title: Reconstituting ring-rafts in bud-mimicking topography of model membranes
Authors: Ryu, Yong-Sang
Lee, In-Ho
Suh, Jeng-Hun
Park, Seung Chul
Oh, Soojung
Jordan, Luke R.
Wittenberg, Nathan J.
Oh, Sang-Hyun
Jeon, Noo Li
Lee, Byoungho
Parikh, Atul N.
Lee, Sin-Doo
Keywords: Membrane
Bud Mimicking Topography
Issue Date: 2014
Source: Ryu, Y.-S., Lee, I.-H., Suh, J.-H., Park, S. C., Oh, S., Jordan, L. R., . . . Lee, S.-D. (2014). Reconstituting ring-rafts in bud-mimicking topography of model membranes. Nature Communications, 5, 4507-. doi:10.1038/ncomms5507
Series/Report no.: Nature Communications
Abstract: During vesicular trafficking and release of enveloped viruses, the budding and fission processes dynamically remodel the donor cell membrane in a protein- or a lipid-mediated manner. In all cases, in addition to the generation or relief of the curvature stress, the buds recruit specific lipids and proteins from the donor membrane through restricted diffusion for the development of a ring-type raft domain of closed topology. Here, by reconstituting the bud topography in a model membrane, we demonstrate the preferential localization of cholesterol- and sphingomyelin-enriched microdomains in the collar band of the bud-neck interfaced with the donor membrane. The geometrical approach to the recapitulation of the dynamic membrane reorganization, resulting from the local radii of curvatures from nanometre-to-micrometre scales, offers important clues for understanding the active roles of the bud topography in the sorting and migration machinery of key signalling proteins involved in membrane budding.
DOI: 10.1038/ncomms5507
Rights: © 2014 Macmillan Publishers Limited. This work is licensed under a Creative Commons AttributionNonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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