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Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Authors: Shi, Meng
Chen, Bing
Mahajan, Divyanshu
Boh, Boon Kim
Zhou, Yan
Dutta, Bamaprasad
Tie, Hieng Chiong
Sze, Siu Kwan
Wu, Geng
Lu, Lei
Keywords: Ragulator
DRNTU::Science::Biological sciences
Small GTPase
Issue Date: 2018
Source: Shi, M., Chen, B., Mahajan, D., Boh, B. K., Zhou, Y., Dutta, B., . . . Lu, L. (2018). Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5. Nature Communications, 9(1), 4987-. doi:10.1038/s41467-018-07444-y
Series/Report no.: Nature Communications
Abstract: The endosome-to-Golgi or endocytic retrograde trafficking pathway is an important post-Golgi recycling route. Here we show that amino acids (AAs) can stimulate the retrograde trafficking and regulate the cell surface localization of certain Golgi membrane proteins. By testing components of the AA-stimulated mTORC1 signaling pathway, we demonstrate that SLC38A9, v-ATPase and Ragulator, but not Rag GTPases and mTORC1, are essential for the AA-stimulated trafficking. Arl5, an ARF-like family small GTPase, interacts with Ragulator in an AA-regulated manner and both Arl5 and its effector, the Golgi-associated retrograde protein complex (GARP), are required for the AA-stimulated trafficking. We have therefore identified a mechanistic connection between the nutrient signaling and the retrograde trafficking pathway, whereby SLC38A9 and v-ATPase sense AA-sufficiency and Ragulator might function as a guanine nucleotide exchange factor to activate Arl5, which, together with GARP, a tethering factor, probably facilitates the endosome-to-Golgi trafficking.
DOI: 10.1038/s41467-018-07444-y
Rights: © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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