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Title: Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions
Authors: Moloudi, Reza
Oh, Steve
Yang, Chun
Teo, Kim Leng
Lam, Alan Tin-Lun
Warkiani, Majid Ebrahimi
Naing, May Win
Keywords: Microcarriers
Mesenchymal Stem Cell
Issue Date: 2018
Source: Moloudi, R., Oh, S., Yang, C., Teo, K. L., Lam, A. T.-L., Warkiani, M. E., & Naing, M. W. (2018). Inertial-based filtration method for removal of microcarriers from mesenchymal stem cell suspensions. Scientific Reports, 8, 12481-.
Series/Report no.: Scientific Reports
Abstract: Rapidly evolving cell-based therapies towards clinical trials demand alternative approaches for efficient expansion of adherent cell types such as human mesenchymal stem cells (hMSCs). Using microcarriers (100–300 µm) in a stirred tank bioreactor offers considerably enhanced surface to volume ratio of culture environment. However, downstream purification of the harvested cell product needs to be addressed carefully due to distinctive features and fragility of these cell products. This work demonstrates a novel alternative approach which utilizes inertial focusing to separate microcarriers (MCs) from the final cell suspension. First, we systematically investigated MC focusing dynamics inside scaled-up curved channels with trapezoidal and rectangular cross-sections. A trapezoidal spiral channel with ultra-low-slope (Tan(α)=0.0375) was found to contribute to strong MC focusing (~300 < Re < ~400) while managing high MC volume fractions up to ~1.68%. Accordingly, the high-throughput trapezoidal spiral channel successfully separated MCs from hMSC suspension with total cell yield~94% (after two passes) at a high volumetric flow rate of ~30 mL/min (Re~326.5).
ISSN: 2045-2322
DOI: 10.1038/s41598-018-31019-y
Rights: © 2018 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
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