Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89150
Title: Identification of arenin, a novel Kunitz-like polypeptide from the skin secretions of Dryophytes arenicolor
Authors: Hernández-Pérez, Jesús
Serra, Aida
Sze, Siu Kwan
Conway, Patricia Lynne
Schlundt, Jørgen
Benavides, Jorge
Keywords: DRNTU::Science::Biological sciences
Amphibians
Skin Secretions
Issue Date: 2018
Source: Hernández-Pérez, J., Serra, A., Sze, S. K., Conway, P. L., Schlundt, J., & Benavides, J. (2018). Identification of arenin, a novel Kunitz-like polypeptide from the skin secretions of Dryophytes arenicolor. International Journal of Molecular Sciences, 19(11), 3644-. doi: 10.3390/ijms19113644
Series/Report no.: International Journal of Molecular Sciences
Abstract: Amphibian skin secretions are enriched with complex cocktails of bioactive molecules such as proteins, peptides, biogenic amines, alkaloids guanidine derivatives, steroids and other minor components spanning a wide spectrum of pharmacological actions exploited for centuries in folk medicine. This study presents evidence on the protein profile of the skin secretions of the canyon tree frog, Dryophytes arenicolor. At the same time, it presents the reverse-phase liquid chromatography isolation, mass spectrometry characterization and identification at mRNA level of a novel 58 amino acids Kunitz-like polypeptide from the skin secretions of Dryophytes arenicolor, arenin. Cell viability assays performed on HDFa, CaCo2 and MCF7 cells cultured with different concentrations of arenin showed a discrete effect at low concentrations (2, 4, 8 and 16 µg/mL) suggesting a multi-target interaction in a hormetic-like dose-response. Further work is required to investigate the mechanisms underlying the variable effect on cell viability produced by different concentrations of arenin.
URI: https://hdl.handle.net/10356/89150
http://hdl.handle.net/10220/47036
ISSN: 1661-6596
DOI: 10.3390/ijms19113644
Rights: © 2018 The Author(s). Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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