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dc.contributor.authorKeogh, Damienen
dc.contributor.authorLam, Ling Ningen
dc.contributor.authorPavagadhi, Shrutien
dc.contributor.authorUmashankar, Shivshankaren
dc.contributor.authorDoyle, Lucinda Elizabethen
dc.contributor.authorMatysik, Artur Stanislawen
dc.contributor.authorMarsili, Enricoen
dc.contributor.authorKline, Kimberly A.en
dc.contributor.authorSong, Yiyangen
dc.contributor.authorNg, Sean Pinen
dc.contributor.authorBoothroyd, Chris B.en
dc.contributor.authorDunny, Gary M.en
dc.contributor.authorLow, Pui Manen
dc.contributor.authorDale, Jennifer L.en
dc.contributor.authorWilliams, Rohan B. H.en
dc.contributor.authorSwarup, Sanjayen
dc.contributor.editorHancock, Lynn E.en
dc.contributor.editorHultgren, Scott J.en
dc.identifier.citationKeogh, D., Lam, L. N., Doyle, L. E., Matysik, A., Pavagadhi, S., Umashankar, S., et al. (2018). Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism. mBio, 9(2), e00626-17-.en
dc.description.abstractEnterococci are important human commensals and significant opportunistic pathogens. Biofilm-related enterococcal infections, such as endocarditis, urinary tract infections, wound and surgical site infections, and medical device-associated infections, often become chronic upon the formation of biofilm. The biofilm matrix establishes properties that distinguish this state from free-living bacterial cells and increase tolerance to antimicrobial interventions. The metabolic versatility of the enterococci is reflected in the diversity and complexity of environments and communities in which they thrive. Understanding metabolic factors governing colonization and persistence in different host niches can reveal factors influencing the transition to biofilm pathogenicity. Here, we report a form of iron-dependent metabolism for Enterococcus faecalis where, in the absence of heme, extracellular electron transfer (EET) and increased ATP production augment biofilm growth. We observe alterations in biofilm matrix depth and composition during iron-augmented biofilm growth. We show that the ldh gene encoding L-lactate dehydrogenase is required for iron-augmented energy production and biofilm formation and promotes EET.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent16 p.en
dc.rights© 2018 Keogh et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license.en
dc.subjectEnterococcus Faecalisen
dc.titleExtracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolismen
dc.typeJournal Articleen
dc.contributor.schoolInterdisciplinary Graduate School (IGS)en
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.contributor.schoolSchool of Chemical and Biomedical Engineeringen
dc.contributor.schoolSchool of Materials Science and Engineeringen
dc.contributor.schoolSchool of Biological Sciencesen
dc.contributor.organizationSingapore Centre for Environmental Life Sciences Engineeringen
dc.contributor.researchSingapore Phenome Centreen
dc.description.versionPublished versionen
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