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https://hdl.handle.net/10356/89155
Title: | Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism | Authors: | Keogh, Damien Lam, Ling Ning Pavagadhi, Shruti Umashankar, Shivshankar Doyle, Lucinda Elizabeth Matysik, Artur Stanislaw Marsili, Enrico Kline, Kimberly A. Song, Yiyang Ng, Sean Pin Boothroyd, Chris B. Dunny, Gary M. Low, Pui Man Dale, Jennifer L. Williams, Rohan B. H. Swarup, Sanjay |
Keywords: | Enterococcus Faecalis Biofilm |
Issue Date: | 2018 | Source: | Keogh, D., Lam, L. N., Doyle, L. E., Matysik, A., Pavagadhi, S., Umashankar, S., et al. (2018). Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism. mBio, 9(2), e00626-17-. | Series/Report no.: | mBio | Abstract: | Enterococci are important human commensals and significant opportunistic pathogens. Biofilm-related enterococcal infections, such as endocarditis, urinary tract infections, wound and surgical site infections, and medical device-associated infections, often become chronic upon the formation of biofilm. The biofilm matrix establishes properties that distinguish this state from free-living bacterial cells and increase tolerance to antimicrobial interventions. The metabolic versatility of the enterococci is reflected in the diversity and complexity of environments and communities in which they thrive. Understanding metabolic factors governing colonization and persistence in different host niches can reveal factors influencing the transition to biofilm pathogenicity. Here, we report a form of iron-dependent metabolism for Enterococcus faecalis where, in the absence of heme, extracellular electron transfer (EET) and increased ATP production augment biofilm growth. We observe alterations in biofilm matrix depth and composition during iron-augmented biofilm growth. We show that the ldh gene encoding L-lactate dehydrogenase is required for iron-augmented energy production and biofilm formation and promotes EET. | URI: | https://hdl.handle.net/10356/89155 http://hdl.handle.net/10220/44793 |
ISSN: | 2161-2129 | DOI: | 10.1128/mBio.00626-17 | Schools: | Interdisciplinary Graduate School (IGS) Lee Kong Chian School of Medicine (LKCMedicine) School of Chemical and Biomedical Engineering School of Materials Science & Engineering School of Biological Sciences |
Organisations: | Singapore Centre for Environmental Life Sciences Engineering | Research Centres: | Singapore Phenome Centre | Rights: | © 2018 Keogh et al. This is an openaccess article distributed under the terms of the Creative Commons Attribution 4.0 International license. | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | SBS Journal Articles |
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Extracellular Electron Transfer Powers Enterococcus faecalis Biofilm Metabolism.pdf | 2.92 MB | Adobe PDF | View/Open |
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