Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89182
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dc.contributor.authorMeleppat, Ratheesh Kumaren
dc.contributor.authorMurukeshan, Vadakke Mathamen
dc.contributor.authorSeah, Leong Keeyen
dc.contributor.authorShearwood, Christopheren
dc.contributor.editorAsundi, Anand K.en
dc.contributor.editorFu, Yuen
dc.date.accessioned2018-12-17T08:30:28Zen
dc.date.accessioned2019-12-06T17:19:41Z-
dc.date.available2018-12-17T08:30:28Zen
dc.date.available2019-12-06T17:19:41Z-
dc.date.issued2015en
dc.identifier.citationMeleppat, R. K., Murukeshan, V. M., Seah, L. K., & Shearwood, C. (2015). Quantification of biofilm thickness using a swept source based optical coherence tomography system. Proceedings of SPIE - International Conference on Optical and Photonic Engineering (icOPEN2015), 9524, 95242L-. doi:10.1117/12.2190106en
dc.identifier.urihttps://hdl.handle.net/10356/89182-
dc.description.abstractOptical coherence tomography (OCT) is a non-invasive, non-contact optical measurement and imaging technique that relies on low coherence interferometry. Apart from bio-imaging applications, the applicability of OCT can be extended to metrological investigations because of the inherent capability of optical interferometry to perform precise measurement with high sensitivity. In this paper, we demonstrate the feasibility of OCT for the measurement of the refractive index and thickness of bacterial biofilm structures grown in a flow cell. In OCT, the depth profiles are constructed by measuring the magnitude and time delay of back reflected light from the scattering sites by means of optical interferometry. The optical distance between scattering points can be obtained by measuring the separation between the point spread functions (PSF) at the respective points in the A-scan data. The refractive index of the biofilm is calculated by measuring the apparent shift in the position of the PSF corresponding to a reference surface, caused by the biofilm growth. In our experiment, the base layer of the flow cell is used as the reference surface. It is observed that the calculated refractive index of the biofilm is close to that of water, and agrees well with the previously reported value. Finally, the physical thickness of the biofilm is calculated by dividing the optical path length by the calculated value of refractive index.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent6 p.en
dc.language.isoenen
dc.rights© 2015 Society of Photo-optical Instrumentation Engineers (SPIE). This paper was published in Proceedings of SPIE - International Conference on Optical and Photonic Engineering (icOPEN2015) and is made available as an electronic reprint (preprint) with permission of Society of Photo-optical Instrumentation Engineers (SPIE). The published version is available at: [http://dx.doi.org/10.1117/12.2190106]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectOptical Frequency Domain Reflectometryen
dc.subjectOptical Coherence Tomographyen
dc.subjectDRNTU::Engineering::Mechanical engineeringen
dc.titleQuantification of biofilm thickness using a swept source based optical coherence tomography systemen
dc.typeConference Paperen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen
dc.contributor.conferenceProceedings of SPIE - International Conference on Optical and Photonic Engineering (icOPEN2015)en
dc.contributor.organizationBiological Process Laboratoryen
dc.contributor.researchCentre for Optical and Laser Engineeringen
dc.identifier.doi10.1117/12.2190106en
dc.description.versionPublished versionen
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