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Title: Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy
Authors: Yam, Gary Hin-Fai
Teo, Ericia Pei-Wen
Setiawan, Melina
Lovatt, Matthew J.
M. Yusoff, Nur Zahirah
Fuest, Matthias
Goh, Bee-Tin
Mehta, Jodhbir Singh
Keywords: Cornea
Corneal Stromal Keratocyte
Issue Date: 2018
Source: Yam, G. H.-F., Teo, E. P.-W., Setiawan, M., Lovatt, M. J., M. Yusoff, N. Z., Fuest, M., et al. (2018). Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy. Journal of Cellular and Molecular Medicine, 22(6), 3119-3132.
Series/Report no.: Journal of Cellular and Molecular Medicine
Abstract: Corneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non‐functional or orthodontic reason and differentiated them towards CSK phenotype using a two‐step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL‐differentiated CSK‐like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14‐day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities.
ISSN: 1582-1838
DOI: 10.1111/jcmm.13589
Rights: © 2018 The Authors (published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine). This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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