Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorYam, Gary Hin-Faien
dc.contributor.authorTeo, Ericia Pei-Wenen
dc.contributor.authorSetiawan, Melinaen
dc.contributor.authorLovatt, Matthew J.en
dc.contributor.authorM. Yusoff, Nur Zahirahen
dc.contributor.authorFuest, Matthiasen
dc.contributor.authorGoh, Bee-Tinen
dc.contributor.authorMehta, Jodhbir Singhen
dc.identifier.citationYam, G. H.-F., Teo, E. P.-W., Setiawan, M., Lovatt, M. J., M. Yusoff, N. Z., Fuest, M., et al. (2018). Postnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapy. Journal of Cellular and Molecular Medicine, 22(6), 3119-3132.en
dc.description.abstractCorneal opacities are a leading cause of global blindness. They are conventionally treated by the transplantation of donor corneal tissue, which is, restricted by a worldwide donor material shortage and allograft rejection. Autologous adult stem cells with a potential to differentiate into corneal stromal keratocytes (CSKs) could offer a suitable choice of cells for regenerative cell therapy. Postnatal periodontal ligament (PDL) contains a population of adult stem cells, which has a similar embryological origin as CSK, that is cranial neural crest. We harvested PDL cells from young adult teeth extracted because of non‐functional or orthodontic reason and differentiated them towards CSK phenotype using a two‐step protocol with spheroid formation followed by growth factor and cytokine induction in a stromal environment (human amnion stroma and porcine corneal stroma). Our results showed that the PDL‐differentiated CSK‐like cells expressed CSK markers (CD34, ALDH3A1, keratocan, lumican, CHST6, B3GNT7 and Col8A2) and had minimal expression of genes related to fibrosis and other lineages (vasculogenesis, adipogenesis, myogenesis, epitheliogenesis, neurogenesis and hematogenesis). Introduction of PDL spheroids into the stroma of porcine corneas resulted in extensive migration of cells inside the host stroma after 14‐day organ culture. Their quiescent nature and uniform cell distribution resembled to that of mature CSKs inside the native stroma. Our results demonstrated the potential translation of PDL cells for regenerative corneal cell therapy for corneal opacities.en
dc.description.sponsorshipNRF (Natl Research Foundation, S’pore)en
dc.description.sponsorshipNMRC (Natl Medical Research Council, S’pore)en
dc.format.extent14 p.en
dc.relation.ispartofseriesJournal of Cellular and Molecular Medicineen
dc.rights© 2018 The Authors (published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine). This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectCorneal Stromal Keratocyteen
dc.titlePostnatal periodontal ligament as a novel adult stem cell source for regenerative corneal cell therapyen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
Appears in Collections:MSE Journal Articles

Google ScholarTM



Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.