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dc.contributor.authorVaschenko, Svetlanaen
dc.contributor.authorRamanenka, Andreyen
dc.contributor.authorKulakovich, Olgaen
dc.contributor.authorMuravitskaya, Alinaen
dc.contributor.authorGuzatov, Dmitryen
dc.contributor.authorLunevich, Anatolyen
dc.contributor.authorGlukhov, Yurien
dc.contributor.authorGaponenko, Sergeyen
dc.identifier.citationVaschenko, S., Ramanenka, A., Kulakovich, O., Muravitskaya, A., Guzatov, D., Lunevich, A., ... Gaponenko, S. (2016). Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids. Procedia Engineering, 140, 57-66. doi : 10.1016/j.proeng.2015.08.1111en
dc.description.abstractA model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times.en
dc.format.extent10 p.en
dc.relation.ispartofseriesProcedia Engineeringen
dc.rights© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license ( Selection and/or peer-review under responsibility of the scientific committee of Symposium 2015 ICMATen
dc.subjectSilver Nanoparticlesen
dc.subjectDRNTU::Engineering::Electrical and electronic engineeringen
dc.subjectPasmon-enhanced Fluorescenceen
dc.titleEnhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloidsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Electrical and Electronic Engineeringen
dc.description.versionPublished versionen
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