Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89233
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dc.contributor.authorVaschenko, Svetlanaen
dc.contributor.authorRamanenka, Andreyen
dc.contributor.authorKulakovich, Olgaen
dc.contributor.authorMuravitskaya, Alinaen
dc.contributor.authorGuzatov, Dmitryen
dc.contributor.authorLunevich, Anatolyen
dc.contributor.authorGlukhov, Yurien
dc.contributor.authorGaponenko, Sergeyen
dc.date.accessioned2018-10-02T09:32:44Zen
dc.date.accessioned2019-12-06T17:20:49Z-
dc.date.available2018-10-02T09:32:44Zen
dc.date.available2019-12-06T17:20:49Z-
dc.date.issued2016en
dc.identifier.citationVaschenko, S., Ramanenka, A., Kulakovich, O., Muravitskaya, A., Guzatov, D., Lunevich, A., ... Gaponenko, S. (2016). Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids. Procedia Engineering, 140, 57-66. doi : 10.1016/j.proeng.2015.08.1111en
dc.identifier.issn1877-7058en
dc.identifier.urihttps://hdl.handle.net/10356/89233-
dc.description.abstractA model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times.en
dc.format.extent10 p.en
dc.language.isoenen
dc.relation.ispartofseriesProcedia Engineeringen
dc.rights© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). Selection and/or peer-review under responsibility of the scientific committee of Symposium 2015 ICMATen
dc.subjectSilver Nanoparticlesen
dc.subjectDRNTU::Engineering::Electrical and electronic engineeringen
dc.subjectPasmon-enhanced Fluorescenceen
dc.titleEnhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloidsen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Electrical and Electronic Engineeringen
dc.identifier.doi10.1016/j.proeng.2015.08.1111en
dc.description.versionPublished versionen
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item.grantfulltextopen-
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