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https://hdl.handle.net/10356/89233
Title: | Enhancement of labeled alpha-fetoprotein antibodies and antigen-antibody complexes fluorescence with silver nanocolloids | Authors: | Vaschenko, Svetlana Ramanenka, Andrey Kulakovich, Olga Muravitskaya, Alina Guzatov, Dmitry Lunevich, Anatoly Glukhov, Yuri Gaponenko, Sergey |
Keywords: | Silver Nanoparticles DRNTU::Engineering::Electrical and electronic engineering Pasmon-enhanced Fluorescence |
Issue Date: | 2016 | Source: | Vaschenko, S., Ramanenka, A., Kulakovich, O., Muravitskaya, A., Guzatov, D., Lunevich, A., ... Gaponenko, S. (2016). Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids. Procedia Engineering, 140, 57-66. doi : 10.1016/j.proeng.2015.08.1111 | Series/Report no.: | Procedia Engineering | Abstract: | A model immunoassay test system is proposed based on metal enhanced photoluminescence to analyze low concentrations of alpha-fetoprotein, a tumor marker. Antigen-antibody reaction was performed on polystyrene plates coated with silver nanoparticles to increase sensitivity of fluorescent immunoassay and signal-to-noise ratio as compared to silver-free system. The proposed test system model uses layer-by-layer assembly approach, LED excitation and nanowatt photodetection set-up, so it is characterized by smaller probe volume, fast analysis and simplicity. The proposed model system offers alpha-fetoprotein detection at concentrations used in clinical practice. Photostability and photoluminescence enhancement for labeled alpha-fetoprotein antibodies on a silver substrate were found to depend on antibodies concentration. Labeled antibodies photostability on substrates was found to deviate within 20% as compared to silver-free samples whereas photoluminescence intensity enhancement is typically in the range of 6-8 times. | URI: | https://hdl.handle.net/10356/89233 http://hdl.handle.net/10220/46198 |
ISSN: | 1877-7058 | DOI: | 10.1016/j.proeng.2015.08.1111 | Schools: | School of Electrical and Electronic Engineering | Rights: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (https://creativecommons.org/licenses/by-nc-nd/4.0/). Selection and/or peer-review under responsibility of the scientific committee of Symposium 2015 ICMAT | Fulltext Permission: | open | Fulltext Availability: | With Fulltext |
Appears in Collections: | EEE Journal Articles |
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Enhancement of Labeled Alpha-fetoprotein Antibodies and Antigen-antibody Complexes Fluorescence with Silver Nanocolloids.pdf | 2.11 MB | Adobe PDF | View/Open |
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