Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89475
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dc.contributor.authorKalailingam, Pazhanichamyen
dc.contributor.authorTan, Hui Bingen
dc.contributor.authorJain, Neerajen
dc.contributor.authorSng, Ming Keaten
dc.contributor.authorChan, Jeremy Soon Kiaten
dc.contributor.authorTan, Nguan Soonen
dc.contributor.authorThanabalu, Thirumaranen
dc.date.accessioned2018-06-06T09:12:17Zen
dc.date.accessioned2019-12-06T17:26:21Z-
dc.date.available2018-06-06T09:12:17Zen
dc.date.available2019-12-06T17:26:21Z-
dc.date.issued2017en
dc.identifier.citationKalailingam, P., Tan, H. B., Jain, N., Sng, M. K., Chan, J. S. K., Tan, N. S., et al. (2017). Conditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammation. Scientific Reports, 7(1), 7311-.en
dc.identifier.issn2045-2322en
dc.identifier.urihttps://hdl.handle.net/10356/89475-
dc.description.abstractNeural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously and regulates actin cytoskeleton remodeling. In order to characterize the role of N-WASP in epidermal homeostasis and cutaneous biology, we generated conditional N-WASP knockout mouse using CK14-cre (cytokeratin 14) to ablate expression of N-WASP in keratinocytes. N-WASPK14KO (N-WASP fl/fl ; CK14-Cre) mice were born following Mendelian genetics suggesting that N-WASP expression in keratinocytes is not essential during embryogenesis. N-WASPK14KO mice exhibited stunted growth, alopecia, dry and wrinkled skin. The dry skin in N-WASPK14KO mice is probably due to increased transepidermal water loss (TEWL) caused by barrier function defects as revealed by dye penetration assay. N-WASPK14KO mice developed spontaneous inflammation in the neck and face 10 weeks after birth. Histological staining revealed thickening of the epidermis, abnormal cornified layer and extensive infiltration of immune cells (mast cells, eosinophils and T-lymphocytes) in N-WASPK14KO mice skin compared to control mice. N-WASPK14KO mice had higher serum levels of IL-1α, TNF-α, IL-6 and IL-17 compared to control mice. Thus our results suggest that conditional N-WASP knockout in keratinocytes leads to compromised skin barrier, higher infiltration of immune cells and hyperproliferation of keratinocytes due to increased production of cytokines highlighting the importance of N-WASP in maintaining the skin homeostasis.en
dc.description.sponsorshipMOE (Min. of Education, S’pore)en
dc.format.extent15 p.en
dc.language.isoenen
dc.relation.ispartofseriesScientific Reportsen
dc.rights© 2017 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en
dc.subjectActinen
dc.subjectGeneticsen
dc.titleConditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammationen
dc.typeJournal Articleen
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en
dc.contributor.schoolSchool of Biological Sciencesen
dc.identifier.doi10.1038/s41598-017-07125-8en
dc.description.versionPublished versionen
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