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Title: Selective effects of miRNA effector protein Argonaute on miRNA processing
Authors: Lee, Li Ming
Keywords: DRNTU::Science::Biological sciences::Molecular biology
Issue Date: 2018
Publisher: Nanyang Technological University
Source: Lee, L. M. (2018). Selective effects of miRNA effector protein Argonaute on miRNA processing. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Since the discovery of miRNAs, studies have been focused on understanding the mechanisms of miRNA biogenesis. However, the molecular/mechanistic function of some miRNA biogenesis factors remain elusive. This project examines a novel role for Argonaute proteins as a factor in regulation of the pre-mir-124 hairpin. Pre-miR-124, but not other pre-miRNAs, was reduced in response to Ago overexpression. This phenomenon was reproducible in mouse and fly cells, suggesting that the mechanism is broadly conserved. Ago-dependent decrease of pre-miR-124 was shown not to be caused by secondary effects of miR-124 target regulation. Experiments using Dicer knockout cells revealed that Ago-induced reduction of pre-mir-124 is a Dicer-dependent phenomenon. Overexpression of Ago2 appeared to result in an increase in the average neurite length of N2a cells. These observations suggest previously unknown roles for the core miRNA biogenesis factors.
DOI: 10.32657/10220/46311
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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