Please use this identifier to cite or link to this item:
|Title:||Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast||Authors:||Miao, Yansong
Yates, John R.
Drubin, David G.
|Issue Date:||2016||Source:||Miao, Y., Han, X., Zheng, L., Xie, Y., Mu, Y., Yates, J. R., & Drubin, D. G. (2016). Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast. Nature Communications, 7,11265-. doi:10.1038/ncomms11265||Series/Report no.:||Nature Communications||Abstract:||Actin cables, composed of actin filament bundles nucleated by formins, mediate intracellular transport for cell polarity establishment and maintenance. We previously observed that metaphase cells preferentially promote actin cable assembly through cyclin-dependent kinase 1 (Cdk1) activity. However, the relevant metaphase Cdk1 targets were not known. Here we show that the highly conserved actin filament crosslinking protein fimbrin is a critical Cdk1 target for actin cable assembly regulation in budding yeast. Fimbrin is specifically phosphorylated on threonine 103 by the metaphase cyclin–Cdk1 complex, in vivo and in vitro. On the basis of conformational simulations, we suggest that this phosphorylation stabilizes fimbrin’s N-terminal domain, and modulates actin filament binding to regulate actin cable assembly and stability in cells. Overall, this work identifies fimbrin as a key target for cell cycle regulation of actin cable assembly in budding yeast, and suggests an underlying mechanism.||URI:||https://hdl.handle.net/10356/89797
|DOI:||10.1038/ncomms11265||Rights:||© 2016 The Author(s) (Published by Nature Publishing Group). This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
Files in This Item:
|Fimbrin phosphorylation by metaphase Cdk1 regulates actin cable dynamics in budding yeast.pdf||5.62 MB||Adobe PDF|
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.