Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89819
Title: Microfiber drug/gene delivery platform for study of myelination
Authors: Ong, William
Lin, Junquan
Bechler, Marie E.
Wang, Kai
Wang, Mingfeng
ffrench-Constant, Charles
Chew, Sing Yian
Keywords: Electrospinning
RNA Interference
DRNTU::Science::Medicine::Biomedical engineering
Issue Date: 2018
Source: Ong, W., Lin, J., Bechler, M. E., Wang, K., Wang, M., ffrench-Constant, C., & Chew, S. Y. (2018). Microfiber drug/gene delivery platform for study of myelination. Acta Biomaterialia, 75152-160. doi:10.1016/j.actbio.2018.06.011
Series/Report no.: Acta Biomaterialia
Abstract: Our ability to rescue functional deficits after demyelinating diseases or spinal cord injuries is limited by our lack of understanding of the complex remyelination process, which is crucial to functional recovery. In this study, we developed an electrospun suspended poly(ε-caprolactone) microfiber platform to enable the screening of therapeutics for remyelination. As a proof of concept, this platform employed scaffold-mediated non-viral delivery of a microRNA (miR) cocktail to promote oligodendrocyte precursor cells (OPCs) differentiation and myelination. We observed enhanced OPCs differentiation when the cells were transfected with miR-219 and miR-338 on the microfiber substrates. Moreover, miRs promoted the formation of MBP+ tubular extensions around the suspended fibers, which was indicative of myelination, instead of flat myelin membranes on 2D substrates. In addition, OPCs that were transfected with the cocktail of miRs formed significantly longer and larger amounts of MBP+ extensions. Taken together, these results demonstrate the efficacy of this functional screening platform for understanding myelination.
URI: https://hdl.handle.net/10356/89819
http://hdl.handle.net/10220/46451
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2018.06.011
Rights: © 2018 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Acta Biomaterialia, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http:dx.doi.org/10.1016/j.actbio.2018.06.011].
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:IGS Journal Articles
LKCMedicine Journal Articles
SCBE Journal Articles

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