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Title: Bifunctional fluorescent/Raman nanoprobe for the early detection of amyloid
Authors: Xia, Yang
Padmanabhan, Parasuraman
Sarangapani, Sreelatha
Gulyás, Balázs
Matham, Murukeshan Vadakke
Keywords: Biosensors
Issue Date: 2019
Source: Xia, Y., Padmanabhan, P., Sarangapani, S., Gulyás, B., & Matham, M. V. (2019). Bifunctional fluorescent/Raman nanoprobe for the early detection of amyloid. Scientific Reports, 9(1), 8497-. doi:10.1038/s41598-019-43288-2
Series/Report no.: Scientific Reports
Abstract: One of the pathological hallmarks of Alzheimer’s disease (AD) is the abnormal aggregation of amyloid beta (Aβ) peptides. Therefore the detection of Aβ peptides and imaging of amyloid plaques are considered as promising diagnostic methods for AD. Here we report a bifunctional nanoprobe prepared by conjugating gold nanoparticles (AuNPs) with Rose Bengal (RB) dye. RB is chosen due to its unique Raman fingerprints and affinity with Aβ peptides. After the conjugation, Raman signals of RB were significantly enhanced due to the surface-enhanced Raman scattering (SERS) effect. Upon binding with Aβ42 peptides, a spectrum change was detected, and the magnitude of the spectrum changes can be correlated with the concentration of target peptides. The peptide/probe interaction also induced a remarkable enhancement in the probes’ fluorescence emission. This fluorescence enhancement was further utilized to image amyloid plaques in the brain slices from transgenic mice. In this study, the RB-AuNPs were used for both SERS-based detection of Aβ42 peptides and fluorescence-based imaging of amyloid plaques. Compared to monofunctional probes, the multifunctional probe is capable to provide more comprehensive pathophysiological information, and therefore, the implementation of such multifunctional amyloid probes is expected to help the investigation of amyloid aggregation and the early diagnosis of AD.
DOI: 10.1038/s41598-019-43288-2
Schools: School of Mechanical and Aerospace Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: Center for Optical and Laser Engineering
Rights: © 2019 The Author(s). This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles

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