Please use this identifier to cite or link to this item:
|Title:||tRNA epitranscriptomics and biased codon are linked to proteome expression in Plasmodium falciparum||Authors:||Dedon, Peter C.
Preiser, Peter Rainer
Ng, Chee Sheng
Babu, Indrakanti Ramesh
Chionh, Yok Hian
|Issue Date:||2018||Source:||Ng, C. S., Sinha, A., Aniweh, Y., Nah, Q., Babu, I. R., Gu, C., . . . Preiser, P. R. (2018). tRNA epitranscriptomics and biased codon are linked to proteome expression in Plasmodium falciparum. Molecular Systems Biology, 14(10), e8009-. doi:10.15252/msb.20178009||Series/Report no.:||Molecular Systems Biology||Abstract:||Among components of the translational machinery, ribonucleoside modifications on tRNAs are emerging as critical regulators of cell physiology and stress response. Here, we demonstrate highly coordinated behavior of the repertoire of tRNA modifications of Plasmodium falciparum throughout the intra‐erythrocytic developmental cycle (IDC). We observed both a synchronized increase in 22 of 28 modifications from ring to trophozoite stage, consistent with tRNA maturation during translational up‐regulation, and asynchronous changes in six modifications. Quantitative analysis of ~2,100 proteins across the IDC revealed that up‐ and down‐regulated proteins in late but not early stages have a marked codon bias that directly correlates with parallel changes in tRNA modifications and enhanced translational efficiency. We thus propose a model in which tRNA modifications modulate the abundance of stage‐specific proteins by enhancing translation efficiency of codon‐biased transcripts for critical genes. These findings reveal novel epitranscriptomic and translational control mechanisms in the development and pathogenesis of Plasmodium parasites.||URI:||https://hdl.handle.net/10356/89867
|DOI:||10.15252/msb.20178009||Rights:||© 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Journal Articles|
Files in This Item:
|tRNA epitranscriptomics and biased codon are linked to proteome expression in Plasmodium falciparum.pdf||1.99 MB||Adobe PDF|
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.