Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89867
Title: tRNA epitranscriptomics and biased codon are linked to proteome expression in Plasmodium falciparum
Authors: Dedon, Peter C.
Preiser, Peter Rainer
Ng, Chee Sheng
Sinha, Ameya
Aniweh, Yaw
Nah, Qianhui
Babu, Indrakanti Ramesh
Gu, Chen
Chionh, Yok Hian
Keywords: DRNTU::Science::Biological sciences
Quantitative Proteomics
Plasmodium Falciparum
Issue Date: 2018
Source: Ng, C. S., Sinha, A., Aniweh, Y., Nah, Q., Babu, I. R., Gu, C., . . . Preiser, P. R. (2018). tRNA epitranscriptomics and biased codon are linked to proteome expression in Plasmodium falciparum. Molecular Systems Biology, 14(10), e8009-. doi:10.15252/msb.20178009
Series/Report no.: Molecular Systems Biology
Abstract: Among components of the translational machinery, ribonucleoside modifications on tRNAs are emerging as critical regulators of cell physiology and stress response. Here, we demonstrate highly coordinated behavior of the repertoire of tRNA modifications of Plasmodium falciparum throughout the intra‐erythrocytic developmental cycle (IDC). We observed both a synchronized increase in 22 of 28 modifications from ring to trophozoite stage, consistent with tRNA maturation during translational up‐regulation, and asynchronous changes in six modifications. Quantitative analysis of ~2,100 proteins across the IDC revealed that up‐ and down‐regulated proteins in late but not early stages have a marked codon bias that directly correlates with parallel changes in tRNA modifications and enhanced translational efficiency. We thus propose a model in which tRNA modifications modulate the abundance of stage‐specific proteins by enhancing translation efficiency of codon‐biased transcripts for critical genes. These findings reveal novel epitranscriptomic and translational control mechanisms in the development and pathogenesis of Plasmodium parasites.
URI: https://hdl.handle.net/10356/89867
http://hdl.handle.net/10220/47710
DOI: 10.15252/msb.20178009
Rights: © 2018 The Authors. This is an open access article under the terms of the Creative Commons Attribution 4.0 License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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