Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89931
Title: Predicting allosteric effects from orthosteric binding in Hsp90-ligand interactions : implications for fragment-based drug design
Authors: Chandramohan, Arun
Krishnamurthy, Srinath
Larsson, Andreas
Nordlund, Paer
Jansson, Anna
Anand, Ganesh S.
Keywords: Orthosteric
DRNTU::Science::Biological sciences
Allosteric
Issue Date: 2016
Source: Chandramohan, A., Krishnamurthy, S., Larsson, A., Nordlund, P., Jansson, A., & Anand, G. S. (2016). Predicting allosteric effects from orthosteric binding in Hsp90-ligand interactions : implications for fragment-based drug design. PLOS Computational Biology, 12(6), e1004840-.
Chandramohan, A., Krishnamurthy, S., Larsson, A., Nordlund, P., Jansson, A., & Anand, G. S. (2016). Predicting allosteric effects from orthosteric binding in Hsp90-ligand interactions : implications for fragment-based drug design. PLOS Computational Biology, 12(6), e1004840-. doi:10.1371/journal.pcbi.1004840
Series/Report no.: PLOS Computational Biology
Abstract: A key question in mapping dynamics of protein-ligand interactions is to distinguish changes at binding sites from those associated with long range conformational changes upon binding at distal sites. This assumes a greater challenge when considering the interactions of low affinity ligands (dissociation constants, KD, in the μM range or lower). Amide hydrogen deuterium Exchange mass spectrometry (HDXMS) is a robust method that can provide both structural insights and dynamics information on both high affinity and transient protein-ligand interactions. In this study, an application of HDXMS for probing the dynamics of low affinity ligands to proteins is described using the N-terminal ATPase domain of Hsp90. Comparison of Hsp90 dynamics between high affinity natural inhibitors (KD ~ nM) and fragment compounds reveal that HDXMS is highly sensitive in mapping the interactions of both high and low affinity ligands. HDXMS reports on changes that reflect both orthosteric effects and allosteric changes accompanying binding. Orthosteric sites can be identified by overlaying HDXMS onto structural information of protein-ligand complexes. Regions distal to orthosteric sites indicate long range conformational changes with implications for allostery. HDXMS, thus finds powerful utility as a high throughput method for compound library screening to identify binding sites and describe allostery with important implications for fragment-based ligand discovery (FBLD).
URI: https://hdl.handle.net/10356/89931
http://hdl.handle.net/10220/46439
ISSN: 1553-734X
DOI: 10.1371/journal.pcbi.1004840
Rights: © 2016 Chandramohan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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