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Title: A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl
Authors: Zhou, Miaomiao
Lee, Bae Hoon
Tan, Lay Poh
Keywords: DRNTU::Engineering::Materials
Microbial Transglutaminase
Enzymatic Crosslinking
Issue Date: 2017
Source: Zhou, M., Lee, B. H., & Tan, L. P. (2017). A dual crosslinking strategy to tailor rheological properties of gelatin methacryloyl. International Journal of Bioprinting, 3(2), 130-137. doi:10.18063/IJB.2017.02.003
Series/Report no.: International Journal of Bioprinting
Abstract: 3D bioprinting is an emerging technology that enables fabrication of three-dimensional organised cellular constructs. One of the major challenges in 3D bioprinting is to develop a material to meet the harsh requirements (cell-compatibility, printability, structural stability post-printing and bio-functionality to regulate cell behaviours) suitable for printing. Gelatin methacryloyl (GelMA) has recently emerged as an attractive biomaterial in tissue engineering because it satisfies the requirements of bio-functionality and mechanical tunability. However, the poor rheological property such as low viscosity at body temperature inhibits its application in 3D bioprinting. In this work, an enzymatic crosslinking method triggered by Ca2+-independent microbial transglutaminase (MTGase) was introduced to catalyse isopeptide bonds formation between chains of GelMA, which could improve its rheological behaviours, specifically its viscosity. By combining enzymatic crosslinking and photo crosslinking, it is possible to tune the solution viscosity and quickly stabilize the gelatin macromolecules at the same time. The results showed that the enzymatic crosslinking can increase the solution viscosity. Subsequent photo crosslinking could aid in fast stabilization of the structure and make handling easy.
DOI: 10.18063/IJB.2017.02.003
Rights: © 2017 Miaomiao Zhou, et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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