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Title: Knowledge gaps in rodent pancreas biology : taking human pluripotent stem cell-derived pancreatic beta cells into our own hands
Authors: Santosa, Munirah Mohamad
Low, Blaise Su Jun
Pek, Nicole Min Qian
Teo, Adrian Kee Keong
Keywords: DRNTU::Science::Biological sciences
Issue Date: 2016
Source: Santosa, M. M., Low, B. S. J., Pek, N. M. Q.,& Teo, A. K. K. (2016). Knowledge Gaps in Rodent Pancreas Biology : Taking Human Pluripotent Stem Cell-Derived Pancreatic Beta Cells into Our Own Hands. Frontiers in Endocrinology, 6, 194-. doi: 10.3389/fendo.2015.00194
Series/Report no.: Frontiers in Endocrinology
Abstract: In the field of stem cell biology and diabetes, we and others seek to derive mature and functional human pancreatic β cells for disease modeling and cell replacement therapy. Traditionally, knowledge gathered from rodents is extended to human pancreas developmental biology research involving human pluripotent stem cells (hPSCs). While much has been learnt from rodent pancreas biology in the early steps toward Pdx1+ pancreatic progenitors, much less is known about the transition toward Ngn3+ pancreatic endocrine progenitors. Essentially, the later steps of pancreatic β cell development and maturation remain elusive to date. As a result, the most recent advances in the stem cell and diabetes field have relied upon combinatorial testing of numerous growth factors and chemical compounds in an arbitrary trial-and-error fashion to derive mature and functional human pancreatic β cells from hPSCs. Although this hit-or-miss approach appears to have made some headway in maturing human pancreatic β cells in vitro, its underlying biology is vaguely understood. Therefore, in this mini-review, we discuss some of these late-stage signaling pathways that are involved in human pancreatic β cell differentiation and highlight our current understanding of their relevance in rodent pancreas biology. Our efforts here unravel several novel signaling pathways that can be further studied to shed light on unexplored aspects of rodent pancreas biology. New investigations into these signaling pathways are expected to advance our knowledge in human pancreas developmental biology and to aid in the translation of stem cell biology in the context of diabetes treatments.
DOI: 10.3389/fendo.2015.00194
Rights: © 2016 Santosa, Low, Pek and Teo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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