Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/89985
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLi, Huaqiongen
dc.contributor.authorWen, Fengen
dc.contributor.authorWang, Xincaien
dc.contributor.authorTan, Lay Pohen
dc.date.accessioned2018-10-29T03:11:40Zen
dc.date.accessioned2019-12-06T17:38:02Z-
dc.date.available2018-10-29T03:11:40Zen
dc.date.available2019-12-06T17:38:02Z-
dc.date.issued2015en
dc.identifier.citationLi, H., Wen, F., Wang, X., & Tan, L. P. (2015). Role of RhoA/Rho kinase signaling pathway in microgroove induced stem cell myogenic differentiation. Biointerphases, 10(2), 021003-. doi:10.1116/1.4916624en
dc.identifier.issn1934-8630en
dc.identifier.urihttps://hdl.handle.net/10356/89985-
dc.description.abstractIn our previous report, the authors have demonstrated that direct laser machined microchannels would trigger upregulation of myogenic markers in human mesenchymal stem cells (hMSCs) through promotion of cell elongation. However, the molecular basis signaling pathways behind this observation remains unclear. In this work, three types of microchannels generated by femtosecond laser were utilized to investigate possible mechanisms behind the induction of hMSCs myogenesis by microchannels. The authors hypothesized that small G-proteins RhoA and Rac1 play a vital role on myogenesis of hMSCs through regulating cytoskeleton rearrangement, via cell tension signaling cascades. The RhoA and Rac1 activities were evaluated for cells cultured on the micropatterned substrates, using a flat unpatterned substrate as control. It was found that significant activation of RhoA GTPase was exhibited for cells cultured on narrow microchannels (20-20-20 and 30-30-20), while no obvious differences were obtained on wide ones (80-30-20). Meanwhile, no significant difference was found for Rac1 activities on all tested groups. To further deduce the role of RhoA signaling pathway in microchannel directed stem cell myogenesis, the effectors of Rho, Rho kinase (ROCK) was chosen to explore how cell shape regulate myogenesis of hMSCs cultured on laser micropatterned substrate. A pharmacological ROCK inhibitor, Y-27632, was used to treat the cells and the effect on RhoA activation was investigated. Our data on the role of RhoA/ROCK in regulating cell myogenic differentiation on lasered microchannels substrates may provide a mechanistic insight on hMSCs fate directed by substrate topography.en
dc.format.extent8 p.en
dc.language.isoenen
dc.relation.ispartofseriesBiointerphasesen
dc.rights© 2015 American Vacuum Society. This paper was published in Biointerphases and is made available with permission of American Vacuum Society. The published version is available at: [http://dx.doi.org/10.1116/1.4916624]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law.en
dc.subjectCell Differentiationen
dc.subjectRho-associated Kinasesen
dc.subjectDRNTU::Engineering::Materialsen
dc.titleRole of RhoA/Rho kinase signaling pathway in microgroove induced stem cell myogenic differentiationen
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science & Engineeringen
dc.identifier.doi10.1116/1.4916624en
dc.description.versionPublished versionen
item.fulltextWith Fulltext-
item.grantfulltextopen-
Appears in Collections:MSE Journal Articles

SCOPUSTM   
Citations 50

4
Updated on Mar 26, 2024

Web of ScienceTM
Citations 50

4
Updated on Oct 24, 2023

Page view(s)

278
Updated on Mar 28, 2024

Download(s) 50

141
Updated on Mar 28, 2024

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.